A novel approach to assess the dynamics of extra-chromosomal circular ribosomal DNA in human cells
- Subject Areas
- Biotechnology, Cell Biology, Genomics
- extrachromosomal circular ribosomal DNA, extrachromosomal DNA, homologous recombination, ageing, genomic mosaic
- © 2014 Leheste et al.
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2014. A novel approach to assess the dynamics of extra-chromosomal circular ribosomal DNA in human cells. PeerJ PrePrints 2:e638v1 https://doi.org/10.7287/peerj.preprints.638v1
Several nutrient-signaling pathways that extend life span have been described in model organisms. Thus, parallel and redundant signaling pathways that are similar across species might be subject to experimental manipulation. Here, we develop a PCR-based technique for testing the hypothesis that mitotic accumulation of extra-chromosomal ribosomal DNA circles might also determine life span in human cells. Using resveratrol, a phytochemical that counters age-related signs, we find treatment-dependent subcellular accumulations of extra-chromosomal 5S ribosomal DNA in human cell lines. These data suggest an association between DNA circles and intrinsic aging and demonstrate the utility of a PCR-based technique for studying the accumulation of dysfunctional molecules that promote senescence.
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