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A novel approach to assess the dynamics of extra-chromosomal circular ribosomal DNA in human cells

, , , , , , ,
1 Department of Biomedical Sciences, NYIT College of Osteopathic Medicine, Old Westbury, NY, United States
DOI
10.7287/peerj.preprints.638v1
Published
Accepted
Subject Areas
Biotechnology, Cell Biology, Genomics
Keywords
extrachromosomal circular ribosomal DNA, extrachromosomal DNA, homologous recombination, ageing, genomic mosaic
Copyright
© 2014 Leheste et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
Cite this article
Leheste JR, Forbes E, DiGregorio K, Katz V, Miceli A, Husko C, Ramos RL, Torres G. 2014. A novel approach to assess the dynamics of extra-chromosomal circular ribosomal DNA in human cells. PeerJ PrePrints 2:e638v1

Abstract

Several nutrient-signaling pathways that extend life span have been described in model organisms. Thus, parallel and redundant signaling pathways that are similar across species might be subject to experimental manipulation. Here, we develop a PCR-based technique for testing the hypothesis that mitotic accumulation of extra-chromosomal ribosomal DNA circles might also determine life span in human cells. Using resveratrol, a phytochemical that counters age-related signs, we find treatment-dependent subcellular accumulations of extra-chromosomal 5S ribosomal DNA in human cell lines. These data suggest an association between DNA circles and intrinsic aging and demonstrate the utility of a PCR-based technique for studying the accumulation of dysfunctional molecules that promote senescence.

Author Comment

This is a submission to PeerJ for review.

Additional Information

Competing Interests

The authors declare they have no competing interests.

Author Contributions

Joerg R Leheste conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Emily Forbes conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Kristin DiGregorio performed the experiments, analyzed the data, reviewed drafts of the paper.

Victoria Katz performed the experiments, reviewed drafts of the paper.

Alyssa Miceli analyzed the data, reviewed drafts of the paper.

Christopher Husko analyzed the data, reviewed drafts of the paper.

Raddy L Ramos wrote the paper, reviewed drafts of the paper.

German Torres analyzed the data, wrote the paper, reviewed drafts of the paper.

Funding

This work has been supported by intramural funding (NYITCOM) to J. R. Leheste. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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A novel approach to assess the dynamics of extra-chromosomal circular ribosomal DNA in human cells
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