MicroRNAs expression profile in CCR6+ regulatory T cells
- Published
- Accepted
- Subject Areas
- Biotechnology, Cell Biology, Allergy and Clinical Immunology, Hematology, Immunology
- Keywords
- CCR6, miRNAs, regulatory T cell, microarray
- Copyright
- © 2014 Zhao et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2014. MicroRNAs expression profile in CCR6+ regulatory T cells. PeerJ PrePrints 2:e471v2 https://doi.org/10.7287/peerj.preprints.471v2
Abstract
Backgroud: CCR6+ CD4+ regulatory T cells (CCR6+Tregs), a distinct Tregs subset, played an important role in various immune diseases. Recent evidence showed that microRNAs (miRNAs) are vital regulators in the function of immune cells. However, the potential role of miRNAs in the function of CCR6+Tregs remains largely unknown. In this study, we detected the expression profile of miRNAs in CCR6+ Tregs. Materials and Methods: The expression profile of miRNAs as well as genes in CCR6+Tregs or CCR6-Tregs from Balb/c mice were detected by microarray. The signaling pathways were analyzed using Keggs pathway library. Results: We found that there were 58 miRNAs significantly upregulated and 62 downregulated up to 2 fold in CCR6+Tregs compared with CCR6-Tregs. Moreover, 1391 genes were observed with 3 fold change and 20 signaling pathways were enriched using Keggs pathway library. Conclusion: The present data firstly showed CCR6+Tregs expressed specific miRNAs pattern, which provide an insight into the role of miRNAs in the biological function of distinct Tregs subsets.
Author Comment
We've made some modifications in the legends of Figure 2 and Figure 3.
Supplemental Information
The relative expression of miR-142 and miR-21 in CCR6 + Tregs
CCR6 + Tregsand CCR6 - Tregs were purified from splenocytes in Balb/c mice by FACSsorting. The relative expression of miR-142 and miR-21 in CCR6 + Tregscells was determined by Realtime PCR assay.
The expression of putative targets of miR-142a in CCR6+Tregs
CCR6 + Tregsand CCR6 - Tregs were purified from splenocytes in Balb/c mice by FACSsorting. The global expression of genes in cells was analyzed by microarrayarray and then the expression of putative target genes was listed (CCR6 - Tregs; CCR6 + Tregs)
