Sequence logos for the novel COI Leray-XT primer set
Read abundances (mean + se) of MOTUs assigned to different taxonomic ranks using COI or 18S
Patterns of abundance of metabarcoding reads per replicate sample
Results presented for COI (upper graph) or 18S (lower graph) in eight different marine littoral communities from NE Atlantic (left) and W Mediterranean (right), and a set of lagoon sediment samples.
Patterns of relative MOTU richness obtained using COI (upper graph) or 18S (lower graph) from the same replicate samples of fig. S4
Pie charts showing the reproducibility of metabarcoding results
Plots correspond to three ecological replicates (upper row), three DNA extraction replicates (middle row) and three PCR replicates (lower row) for COI (left) and 18S (right). Arrows indicate which replicate is used in the next level of study.
nMDS plots showing the ordination patterns of Bray-Curtis dissimilarities calculated from read abundances (fourth root transformed)
Data correspond to all MOTUs detected in eight different marine littoral communities and a set of sediment samples from a tidal lagoon. The size fractions (A, B and C) are indicated. Analyses from COI (left) and 18S (right) are shown.
Summary of the metabarcoding pipelines for 18S and COI.
Summary of reference sequences included in the databases used for taxonomic assignment of COI and 18S with ecotag.