High quality draft genome of Brucella abortus strain Col-B012, isolated from a Holstein cattle in Nariño Colombia, brings new insights into the diagnosis and the epidemiology of biovar 4 strains
- Published
- Accepted
- Subject Areas
- Agricultural Science, Genomics
- Keywords
- Brucellosis, biovar, genome, diagnosis, coparative genomics
- Copyright
- © 2017 Pacheco-Montealegre et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. High quality draft genome of Brucella abortus strain Col-B012, isolated from a Holstein cattle in Nariño Colombia, brings new insights into the diagnosis and the epidemiology of biovar 4 strains. PeerJ Preprints 5:e3416v1 https://doi.org/10.7287/peerj.preprints.3416v1
Abstract
Brucellosis is a commonly diagnosed zoonosis that causes infertility and abortion in cattle, it is acquired from handling of infected animals or consuming contaminated milk or milk products. In Colombia, it is classified as prevention and control disease, despite its relevance little is known about the origin, epidemiology and the genetic constituents of the strains circulating in dairy farms. Here we present the draft genome of B. abortus Col-B012, an isolate obtained from a female Holstein belonging to a dairy farm in Nariño, Colombia. This genome comprises 3,234,714 bp and 3,018 predicted protein-encoding genes. Using comparative genomics and phylogenetic analysis, we found that the strain Col-B012 clustered with known biovar 4 variants. The analysis of the core genes allowed the identification of polymorphisms only present in biovar 4 genomes, these alleles might be associated with the phenotypic and pathogenic characteristics of the group and are proposed as possible targets for identification by PCR. The sequencing of B. abortus Col-B012 genome provides important insights to improve the diagnosis and the epidemiology of this disease and represents the first report of the biovar 4 in Colombia.
Author Comment
High quality version of the manuscript