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Supplemental Information

Peptide sequences for differentially expressed proteins identified at the two-week sampling time

The 13 and 25 unique proteins over-expressed after two weeks by samples of the control (C) and high temperature (H) treatments, respectively, were included. Additional details of the sequenced proteins can be found in the main text (Table 2). The compartment of origin has been mentioned next to the spot number except when it could not be determined. Sym=Symbiodinium.

DOI: 10.7287/peerj.preprints.3252v1/supp-1

Peptide sequences for the differentially expressed proteins (DEPs) identified at the four-week sampling time

The 8 and 4 proteins over-expressed after four weeks by samples of the control (C) and high temperature (H) treatments, respectively, were included. Additional details of the sequenced proteins can be found in the main text (Table 3). No unique DEPs were identified from spots H5 and H6. The compartment of origin has been included next to the spot number. Sym=Symbiodinium.

DOI: 10.7287/peerj.preprints.3252v1/supp-2

Peptide sequences for the differentially expressed proteins (DEPs) identified at the eight-week sampling time

The 6 and 19 proteins over-expressed after four weeks by samples of the control (C) and high temperature (H) treatments, respectively, were included. Additional details of the sequenced proteins can be found in the main text (Table 4). The compartment of origin has been included next to the spot number. Sym=Symbiodinium. bact=bacterial.

DOI: 10.7287/peerj.preprints.3252v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Anderson B Mayfield conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Yi-Jyun Chen performed the experiments.

Chi-Yu Lu performed the experiments, contributed reagents/materials/analysis tools.

Chii-Shiarng Chen contributed reagents/materials/analysis tools.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

A permit for coral collection was issued by Taiwan's Kenting National Park (0992900842).

Data Deposition

The following information was supplied regarding data availability:

All data files obtained from the mass spectrometer can be downloaded from the transcriptome+proteome server for the model coral used in this work (Pocillopora acuta): http://symbiont.iis.sinica.edu.tw/coral_pdltte/static/html/index.html#stat. Also, the protein sequences have all been included in supplemental tables.

Funding

This work was funded by a grant from the United States National Science Foundation (OISE-0852960 to ABM). ABM was also funded by a postdoctoral research fellowship from the Khaled bin Sultan Living Oceans Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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