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Structure, dynamics and predicted functional ecology of the gut microbiota of the blue (Haliotis fulgens) and yellow (H. corrugata) abalone from Baja California Sur, Mexico

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Structure, dynamics and predicted functional ecology of the gut microbiota of the blue (Haliotis fulgens) and… https://t.co/1h3iB8z8ZU
Structure, dynamics and predicted functional ecology of the gut microbiota of the blue (Haliotis fulgens) and… https://t.co/gTN5DxVwkN
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Supplemental Information

Exclusive bacterial species

OTUs showing differential abundances between yellow (HC) and blue (HF) abalone with statistical and biological significance obtained from LEfSe analyses. OTUs are ranked according to its effect size (LDA score).

DOI: 10.7287/peerj.preprints.3233v1/supp-1

Taxonomic comparison

Non-parametric multidimensional scaling (MDS) of Sorensen similarity index based on presence and absence of OTUs assembled at 97% similarity cut-off of the gut microbiota of Haliotis fulgens (HF) and H. corrugata (HC).

DOI: 10.7287/peerj.preprints.3233v1/supp-2

Genetic groups interactions

Regression of the average Jaccard distance based on phylogenetic groups of bacterial OTUs (10% similarity) as a function of their abundance –log scale – (HF: Haliotis fulgens, HC: Haliotis corrugata).

DOI: 10.7287/peerj.preprints.3233v1/supp-3

PICRUSt correction

Ranks of the KO genes according to their KO count number (log scale) obtained with the script categorize by function in PICRUSt.

DOI: 10.7287/peerj.preprints.3233v1/supp-4

Classified OTUs assignement

Reads number for each detected OTU.

DOI: 10.7287/peerj.preprints.3233v1/supp-5

PICRUSt identified KOs genes

Count number for each identified gene

DOI: 10.7287/peerj.preprints.3233v1/supp-6

PICRUSt identified metabolic functions

Count number for each identified metabolic function.

DOI: 10.7287/peerj.preprints.3233v1/supp-7

Bacterial species competitive pressures

Average value of Jaccard distance for each OTU detected in yellow (HC) and blue abalone (HF).

DOI: 10.7287/peerj.preprints.3233v1/supp-8

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Francesco Cicala conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, collected samples.

José Alejandro Cisterna-Céliz performed the experiments, analyzed the data, prepared figures and/or tables.

James Douglas Moore analyzed the data, wrote the paper, reviewed drafts of the paper.

Axayácatl Rocha-Olivares conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

NR_118116

Funding

This investigations was funded by grants SAGARPA-CONACYT 2011-C01-163322, UC Mexus-CONACYT CN-14-14, and Fund for Scientific Research and Technological Development of CICESE FID-2012-01. The first author benefited from a graduate fellowship from CONACYT to support his Ph.D. program in Marine Ecology at Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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