Suppl. material 1 - Table S1
Primers evaluated in this study
Suppl. material 2: Figure S1
Developed fusion primers for fwh1 and fwh2 on the Illumina high throughput sequencing platform.
Suppl. material 3: Figure S2
Overview of similarity of used inline tags for the fwh1 and fwh2 fusion primers.
Suppl. material 4: Table S2
Overview of the three Romanian macrozoobenthos sampling sites (Z2, L2, R2).
Suppl. material 5: Figure S3
Overview of the macroinvertebrates composition of the three sample sites in Romania.
Suppl. material 6: Table S3
Overview of used tagging combinations for sample multiplexing for sequencing.
Suppl. material 7: Figure S4
Gradient PCR optimisation for the fwh primer sets.
Suppl. material 8: Script S1
JAMP metabarcoding pipeline (used R commands).
Suppl. material 9: Figure S5
Number of raw sequences obtained for each sample after demultiplexing.
Suppl. material 10: Table S4
OTU table for the 52 taxa mock samples sequenced with the fwh1 and fwh2 primer set.
Suppl. material 11: Figure S6
Proportion of shared reads between the two replicates for DceM amplified with the fwh1 primer set.