Suppl. material 1: Figure S1
Developed fusion primers for fwh1 and fwh2 on the Illumina high throughput sequencing platform.
Suppl. material 2: Figure S2
Overview of similarity of used inline tags for the fwh1 and fwh2 fusion primers.
Suppl. material 3: Table S1
Overview of the three Romanian macrozoobenthos sampling sites (Z2, L2, R2).
Suppl. material 4: Figure S3
Overview of the macroinvertebrates composition of the three sample sites in Romania.
Suppl. material 5: Table S2
Overview of used tagging combinations for sample multiplexing for sequencing.
Suppl. material 6: Figure S4
Gradient PCR optimisation for the fwh primer sets.
Suppl. material 7: Script S1
JAMP metabarcoding pipeline (used R commands).
Suppl. material 8: Figure S5
Number of raw sequences obtained for each sample after demultiplexing.
Suppl. material 9: Table S3
OTU table for the 52 taxa mock samples sequenced with the fwh1 and fwh2 primer set.
Suppl. material 10: Figure S6
Proportion of shared reads between the two replicates for DceM amplified with the fwh1 primer set.