Video S1. MicroCT rendering (rostro-cranial coronal cross sectioning) of the bill and tongue of an Anna’s Hummingbird, overlaying TEM micrographs
This virtual model of the internal three-dimensional architecture help us to understand the fit between bill and tongue. Additionally, merging microCT, light, and electron microscopy allows us to understand the key morphological features for the tongue functioning linking them across spatial scales. Known objects are placed at the same scale at the different zoom levels in order to contextualize the structures shown.
Video S2. Control of the setup to film post-mortem tongue fluid interactions
Under a dissecting microscope (cf. Fig S2), this control system emulates reciprocating tongue movements and allows for fine control of the distance to the nectar surface using micromanipulators. I coupled a high-speed camera to capture the details of tongue fluid interactions (e.g. Video S3).
Video S3. High-speed recording of post-mortem tongue expansive filling
A slow motion (165 times slower than real time) video of the lateral view of a dissected Ruby-throated Hummingbird focusing on the bill tip – tongue – nectar interaction. The tongue protraction is controlled by micromanipulators (Video S2). Footage obtained under a dissecting microscope (cf. Fig S2) with a flat surface mirror to achieve the side view.
Video S4. Recording of post-mortem tongue fluid trapping
A real-time video of the dorsal view of a deceased Ruby-throated Hummingbird focusing on the bill tip – tongue – nectar interaction. Footage obtained under a dissecting microscope immersing the tongue in a nectar reservoir to appreciate the instantaneous change in shape when the tongue transitions form air (out of focus) to nectar (in focus) and vice versa.
Video S5. Three-dimensional digital rendering of a microCT scan of the skull of a Ruby-throated Hummingbird
This spinning reconstruction makes it possible to follow and visualize the elongated epibranchials surrounding the skull. In this case, the tongue is retracted inside the bill.