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Guo C, Peng X, Zheng X, Wang X, Wang R, Huang Z, Yang Z.2019. Comparison of the bacterial abundance and diversity in the Leptocybe invasa Fisher & La Salle (Hymenoptera: Eulophidae) between both sexes. PeerJ Preprints7:e27826v1https://doi.org/10.7287/peerj.preprints.27826v1
Background. Insects harbor a myriad of microorganisms, many of which can affect the sex ratio and manipulate the reproduction of the host. Leptocybe invasa is an invasive pest that causes serious damage to eucalyptus plantations, and both female-biased sex ratios and thelytokous parthenogenesis in L. invasa contribute to the rapid invasion and fast growth of the population. However, the interior bacterial composition and abundance of L. invasa and the differences between both sexes remain unclear.
Results. The Illumina MiSeq platform was used to compare the composition of the bacterial community in adult females and males by sequencing with variation in the V3-V4 region of the 16S ribosomal DNA gene. The results showed that 1320 operational taxonomic units (OTUs) were obtained in total. These OTUs were annotated into 24 phyla, 71 classes, 130 orders, 245 families and 501 genera. At the genus level, the dominant bacteria in females and males was Rickettsia and Rhizobium, respectively.
Conclusion. The bacteria living in L. invasa adult females and males had high diversity. There were differences in the bacterial community in L. invasa between both sexes, and the bacterial diversity in male adults was more abundant than that in female adults. This study presents a comprehensive comparison of bacterial communities living in L. invasa between sexes, which plays a significant role in reproductive strategy, sex regulation and the invasive mechanism of L. invasa and provides a basis for follow-up studies on the coevolution and interaction between L. invasa and its predominant bacteria.
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Basic information of high-throughput sequencing based on bacteria 16S rDNA in the Leptocybe invasa