Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon

Yong Zhou; Yuan Cheng; Chunpeng Wan; Youxin Yang; Jinyin Chen

doi:10.7287/peerj.preprints.27819v1

Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon

Yong Zhou^1,2, Yuan Cheng³, Chunpeng Wan⁴, Youxin Yang ^1,4, Jinyin Chen ^1,4,5

1 Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, Jiangxi, China

2 College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, Jiangxi province, China

3 Zhejiang Academy of Agricultural Sciences, State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Hanghzou, Zhejiang, China

4 Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi, China

5 Pingxiang University, Pingxiang, Jiangxi province, China

DOI: 10.7287/peerj.preprints.27819v1

Published: 2019-06-24
Accepted: 2019-06-24

Subject Areas: Agricultural Science, Genomics
Keywords: Watermelon, Dof, Phylogenetic analysis, Expression profile, Abiotic stress

Copyright: © 2019 Zhou et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.

Cite this article: Zhou Y, Cheng Y, Wan C, Yang Y, Chen J. 2019. Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon. PeerJ Preprints 7:e27819v1 https://doi.org/10.7287/peerj.preprints.27819v1

Abstract

The plant DNA-binding with one finger (Dof) gene family is a class of plant-specific transcription factors that play vital roles in many biological processes and response to stresses. In the present study, a total of 36 ClDof genes were identified in the watermelon genome, which were unevenly distributed on 10 chromosomes. Phylogenetic analysis showed that the ClDof proteins could be divided into nine groups, and the members in a particular group had similar motif arrangement and exon-intron structure. We then analyzed the expression patterns of nine selected ClDof genes in eight specific tissues by qRT-PCR, and the results showed that they have tissue-specific expression patterns. We also evaluated the expression levels of the nine selected ClDof genes under salt stress and ABA treatments using qRT-PCR, and they showed differential expression under these treatments, suggesting their important roles in stress response. Taken together, our results provide a basis for future research on the biological functions of Dof genes in watermelon.