Multiple propane gas flow rates procedure to determine accuracy and linearity of indirect calorimetry systems : An experimental assessment of a method.
A peer-reviewed article of this Preprint also exists.
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Abstract
Objective: Indirect calorimetry (IC) systems measure the fractions of expired carbon dioxide (FECO2) and oxygen (FEO2) recorded at the mouth in order to estimate whole body energy production (EP). The fundamental principal of IC relates to oxidative mechanisms, expressed as rate of oxygen uptake (V̇O2) and carbon dioxide production (V̇CO2). From these volumes we calculate energy production and respiratory exchange ratio which is used to estimate substrate utilization rates. The accuracy of IC systems is critical to detect small changes in respiratory gas exchanges. The aim of this technical report was to assess the accuracy and linearity of IC systems using multiple propane gas flow rates procedure. Approach: A series of propane gas with different flow rates and ventilation rates were run on three different IC systems. The actual experimental V̇O2 and V̇CO2 were calculated and compared to stoichiometry theoretical values. Results: showed a linear relationship between gas volumes (V̇O2 and V̇CO2) and propane gas flows (99.6%, 99.2%, 94.8% for the Sable, Moxus, and Jaeger metabolic carts, respectively). In terms of system error, Jaeger system had significantly (p < 0.001) greater V̇O2 (M = -0.057, SE = 0.004), and V̇CO2 (M = -0.048, SE = 0.002) error compared to either the Sable (V̇O2, M = 0.044, SE = 0.004; V̇CO2, M = 0.024, SE = 0.002) or the Moxus (V̇O2, M = 0.046, SE = 0.004; V̇CO2, M = 0.025, SE = 0.002) metabolic carts. There were no significant differences between the Sable or Moxus metabolic carts. Conclusion: The multiple flow rates approach permitted the assessment of linearity of IC systems in addition to determining the accuracy of fractions of expired gases.
Cite this as
2019. Multiple propane gas flow rates procedure to determine accuracy and linearity of indirect calorimetry systems : An experimental assessment of a method. PeerJ Preprints 7:e27550v1 https://doi.org/10.7287/peerj.preprints.27550v1note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
Author comment
This method article describes a complementary propane gas calibration procedure to assess accuracy and linearity of indirect calorimetry (IC) systems. The IC systems output must yield accurate fraction of gases for the computation of V̇O2 and V̇CO2 and EP in the field of medicine, nutrition, and exercise sciences. We recommend using the described propane gas mass flow calibration procedure to assess the linearity of IC sensors responses.
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Competing Interests
There are no competing interests
Author Contributions
Mohammad Ismail conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.
Alsubheen A Sana'a contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.
Angela Loucks-Atlinson analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.
Matthew Atkinson analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.
Liam P Kelly conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.
Tim Alkanani conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.
Fabien Basset conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft, supervision of Mr. Mohammad Ismail Master's thesis.
Data Deposition
The following information was supplied regarding data availability:
https://www.dropbox.com/sh/jef8agrr954rurs/AACHFFSfCpJ9DCW0wtAaMLlPa?dl=0
Funding
This work was supported by the School of Human Kinetics and Recreation The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
