Comparative transcriptome analyses of a late-ripening mandarin mutant and its original cultivar reveals gene expression profiling associated with citrus fruit ripening
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Abstract
Characteristics of late maturity in fruits are good agronomic traits for extending the harvest period and marketing time. However, underlying molecular basis of the late-maturing mechanism in fruit is largely unknown. In this study, RNA sequencing (RNA-Seq) technology was used to identify differentially expressed genes (DEGs) related to late-maturing characteristics from a late-maturing mutant ‘Huawan Wuzishatangju’ (HWWZSTJ) (Citrus reticulata Blanco) and its original line ‘Wuzishatangju’ (WZSTJ). A total of approximately 17.0 Gb and 84.2 M paried-end reads were obtained. DEGs were significantly enriched in the pathway of photosynthesis, phenylpropanoid biosynthesis, carotenoid biosynthesis, chlorophyll and abscisic acid (ABA) metabolism. Thirteen candidate transcripts related to chlorophyll metabolism, carotenoid biosynthesis and ABA metabolism were analyzed using real-time quantitative PCR (qPCR) at all fruit maturing stages of HWWZSTJ and WZSTJ. Chlorophyllase (CLH) and divinyl reductase (DVR) from chlorophyll metabolism, phytoene synthase (PSY) and capsanthin/capsorubin synthase (CCS) from carotenoid biosynthesis, abscisic acid 8'-hydroxylase (AB1) and 9-cis-epoxycarotenoid dioxygenase (NCED1) from ABA metabolism were cloned and analyzed. The expression pattern of NCED1 indicates its role in the late-maturing characteristics of HWWZSTJ. There were 270 consecutive bases missing in HWWZSTJ in comparison with full-length sequences of NCED1 cDNA from WZSTJ. Those results suggested that NCED1 might play an important role in late maturity of HWWZSTJ. This study provides new information of complex process that results in late maturity of Citrus fruit at the transcriptional level.
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2017. Comparative transcriptome analyses of a late-ripening mandarin mutant and its original cultivar reveals gene expression profiling associated with citrus fruit ripening. PeerJ Preprints 5:e2744v2 https://doi.org/10.7287/peerj.preprints.2744v2note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
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The manuscript has been revised as per reviewers' comments.
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Wang Lu performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Hua Qingzhu performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Ma Yuewen performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Hu Guibing analyzed the data, reviewed drafts of the paper.
Qin Yonghua conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Data Deposition
The following information was supplied regarding data availability:
The raw data has been supplied as a supplementary file.
Funding
This work was supported by the Science and Technology Planning Project of Guangdong Province (2010B020305007), Key Laboratory of Innovation and Utilization for Germplasm Resources in Horticultural Crops in Southern China of Guangdong Higher Education Institutes, the South China Agricultural University (No. KBL11008). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.