An ADAR1 inducer attenuated the effects of social isolation on depressive-like behavior and ADAR1 (p110) in BALB/c mice
Author and article information
Abstract
Introduction
Social isolation induces depressive-like behavior in animals and humans by impacting RNA editing, but the detailed mechanisms are still unknown. The purpose of this study was to explore how an ADAR1 (RNA-editing enzyme) inducer and inhibitor may impact the isolation-induced depressive-like behavior of mice and to identify new therapeutic targets for the development of an effective solution for the recovery from depressive-like behavior in socially isolated animals and humans.
Methods
Twenty-one-day-old BALB/c mice with and without isolation treatment were evaluated for depressive-like behavior by open-field tests, tail suspension tests, and forced swimming tests. Immunohistochemistry and Western blots were used to measure the immunoreactivity and protein expression of ADAR1 (p110). In addition, the isolated mice were treated with an ADAR1 inducer (IFN-γ) or inhibitor (EHNA). The performance of both treatments on the behavior of and ADAR1 (p110) expression in isolated mice was examined.
Results
Both the immunoreactivity and protein expression of ADAR1 (p110) in the prefrontal cortex decreased in isolated BALB/c mice with depressive-like behavior compared to those of the age-matched, gregarious BALB/c mice. Additionally, the treatments with ADAR1 inducer or inhibitor improved or aggravated depressive-like behavior in isolated mice, respectively. Furthermore, the ADAR1 inducer returned the immunoreactivity and protein expression of ADAR1 (p110) back to the normal level.
Conclusion
The ADAR1 inducer attenuated the effects of social isolation on depressive-like behavior and ADAR1 (p110) in BALB/c mice.
Cite this as
2018. An ADAR1 inducer attenuated the effects of social isolation on depressive-like behavior and ADAR1 (p110) in BALB/c mice. PeerJ Preprints 6:e27413v1 https://doi.org/10.7287/peerj.preprints.27413v1Author comment
This is a submission to PeerJ for review.
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Ying Xue performed the experiments, analyzed the data.
Weizhi Yu performed the experiments, analyzed the data, prepared figures and/or tables.
Jinying Li analyzed the data, prepared figures and/or tables.
Xu Hong performed the experiments.
Xiaonan Zhang performed the experiments.
Huairui Li performed the experiments.
Xiuli Zhai performed the experiments.
Ye Gao contributed reagents/materials/analysis tools.
Lin Gui contributed reagents/materials/analysis tools.
Deqin Yu contributed reagents/materials/analysis tools.
Zhaoyang Xiao conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.
Yiyuan Tang authored or reviewed drafts of the paper.
Shengming Yin conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.
Animal Ethics
The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):
All experimental procedures were approved by the Tab of Animal Experimental Ethical Inspection.
Number: L20140022
Patent Disclosures
The following patent dependencies were disclosed by the authors:
Shengming Ying
Number:201810220277.3
Dates: 2018.03.16
Data Deposition
The following information was supplied regarding data availability:
The raw measurements are provided in the supplementary files.
Funding
This study was supported by the National Natural Science Foundation of China (31201724 and 81471373), China scholarship council (201408210227). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
