Figure 1: Tissues used in this study. Samples were collected as described in the Materials and Methods section.
(A) Callus cells. (B) An emerging tidbit. (C) A young inflorescence. (D) An inflorescence. (E) Mature inflorescence. (F) Developing seed pods. (G) Mature seed pods. (H) Roots. (I) A germinated seed. (J) Young stems. (K) Stems. (L) Mature stems. (M) A young compound leaf. (N) A mature compound leaf. Bar = 1 cm.
Figure 2: STRUCTURE analysis of the genetic structures of five vetch sainfoin
Six different colors represent six different clusters. Pink, cluster 1; Green, cluster 2; Cyan, cluster 3; Blue, cluster 4; Yellow, cluster 5; and Red, cluster 6. Genetic structure of eight individuals in each of the five sainfoin sample populations is inferred by STRUCTURE using the EST-SSR markers dataset.
Figure 3: Phylogenic relationships among the 40 wild sainfoin individuals
The phylogeny tree was constructed using a neighbor-joining dendrogram in the Darwin software. The starting dataset was represented by the 61 best EST-SSRs. I-V, group number representing five different sampling locations. 1-8, sample number representing eight individual samples in the same group.
Figure 4: Comparative analysis of the DNA fragment peak spectrum for two selected EST-SSR loci among the same unit of Onobrychis viciifolia
Table1 Summary of the de novo assembled sainfoin EST-SSRs
Table 2: Functional annotation of sainfiontranscriptome
Supplemental Figure S1: Length distribution of all unigenes
Supplemental Figure S2: COG analysis of the unigene sequences
Supplemental Figure S3: Summary of GO analysis of the unigene sequences
The y-axis on the right indicates the number of genes in a category. The y-axis on the left indicates the number of genes in a specific category.
Supplemental Figure S4: Statistics of the EST-SSR length distribution
Supplemental Figure S5: Gene ontology (GO) term enrichment status for SSR repeat-containing unigenes in sainfoin
Supplemental Figure S6: Variations of EST-SSR markers by PCR using the PCR primer set Vo61 and Vo157 and the 40 wild sainfoin individuals collected from the five different geographic locations
The letter M denotes a molecular marker of 300 and 200bp size (top to bottom).The red triangle indicates the individual plant used to cut the polyacrylamide gel, Vo61selected No. 2 individual from site 2, Vo157 selected No.1 individual from site 2.