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Th2 cytokine bias induced by silver nanoparticles in peripheral blood mononuclear cells of common bottlenose dolphins (Tursiops truncatus)

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122 days ago
Th2 cytokine bias induced by silver nanoparticles in peripheral blood mononuclear cells of common bottlenose dolphins (Tursiops truncatus) https://t.co/ANXY5LrxkX
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Supplemental Information

The size distribution and zeta pontential of the 100 and 500 μg/ml C-AgNP20 in 2 mM citrate buffer (pH 7.4)

DOI: 10.7287/peerj.preprints.27049v1/supp-1

Cytotoxicity of of C-AgNP20 in cPBMCs with or without ConA stimulation

DOI: 10.7287/peerj.preprints.27049v1/supp-2

qPCR efficiencies of each primer set (ct values)

DOI: 10.7287/peerj.preprints.27049v1/supp-3

qPCR efficiencies of each primer set

DOI: 10.7287/peerj.preprints.27049v1/supp-4

Time kinetics of mRNA expression levels of selected cytokines of cPBMCs

The cPBMCs with 0 h incubation were used as control for the calculation of cytokine expression level by △△CT method. In addition, the geometric means of two housekeeping genes (HKGs), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and β2-microglobulin (B2M), of each samples were determined and used to normalize the the expression levels of target genes. The numbers in the sheet were the expression levels.

DOI: 10.7287/peerj.preprints.27049v1/supp-5

Effects of C-AgNP20 on mRNA expression levels of selected cytokines of cPBMCs (4 h)

The cPBMCs with 4 h incubation without C-AgNP20 treatment were used as control for the calculation of cytokine expression level by △△CT method. In addition, the geometric means of two HKGs, GAPDH and B2M, of each samples were determined and used to normalize the the expression levels of target genes

DOI: 10.7287/peerj.preprints.27049v1/supp-6

Effects of C-AgNP20 on mRNA expression levels of selected cytokines of cPBMCs (24 h)

The cPBMCs with 24 h incubation without C-AgNP20 treatment were used as control for the calculation of cytokine expression level by △△CT method. In addition, the geometric means of two HKGs, GAPDH and B2M, of each samples were determined and used to normalize the the expression levels of target genes.

DOI: 10.7287/peerj.preprints.27049v1/supp-7

Additional Information

Competing Interests

Chieh Lo is the veterinarian of Farglory Ocean Park, Hualien, Taiwan.

Author Contributions

Wen-Ta Li conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Lei-Ya Wang performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Hui-Wen Chang conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Wei-Cheng Yang conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Chieh Lo performed the experiments, authored or reviewed drafts of the paper, approved the final draft, collected the blood samples from captive dolphins.

Victor Fei Pang conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Meng-Hsien Chen conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Chian-Ren Jeng conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All procedures involving animals were conducted in accordance with international guidelines, and the protocol had been reviewed and approved by the Council of Agriculture of Taiwan (Approval number 1051700175).

Data Deposition

The following information was supplied regarding data availability:

Raw data have been uploaded on the File page.

The raw data are provided in the Supplemental Files.

Funding

The study was supported by the Ministry of Science & Technology, Taiwan under Grant MOST 106-2313-B-002-054. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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