This is a submission to PeerJ for review.
The cPBMCs with 0 h incubation were used as control for the calculation of cytokine expression level by △△CT method. In addition, the geometric means of two housekeeping genes (HKGs), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and β2-microglobulin (B2M), of each samples were determined and used to normalize the the expression levels of target genes. The numbers in the sheet were the expression levels.
The cPBMCs with 4 h incubation without C-AgNP20 treatment were used as control for the calculation of cytokine expression level by △△CT method. In addition, the geometric means of two HKGs, GAPDH and B2M, of each samples were determined and used to normalize the the expression levels of target genes
The cPBMCs with 24 h incubation without C-AgNP20 treatment were used as control for the calculation of cytokine expression level by △△CT method. In addition, the geometric means of two HKGs, GAPDH and B2M, of each samples were determined and used to normalize the the expression levels of target genes.
Chieh Lo is the veterinarian of Farglory Ocean Park, Hualien, Taiwan.
The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):
All procedures involving animals were conducted in accordance with international guidelines, and the protocol had been reviewed and approved by the Council of Agriculture of Taiwan (Approval number 1051700175).
The following information was supplied regarding data availability:
Raw data have been uploaded on the File page.
The raw data are provided in the Supplemental Files.