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An intermediate state of bacterial ribosome dissociation is fixed by yeast mitochondrial initiation factor 3

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An intermediate state of bacterial ribosome dissociation is fixed by yeast mitochondrial initiation factor 3 https://t.co/JusSbuZrkF
175 days ago
An intermediate state of bacterial ribosome dissociation is fixed by yeast mitochondrial initiation factor 3 https://t.co/AwCxVgmS7J
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Supplemental Information

Raw data for Fig2D (initial scan of the Western-blotting results)

DOI: 10.7287/peerj.preprints.26997v1/supp-5

Initial modeling of Aim23p binding to 16S rRNA of E.coli

Aim23p is in green. Long unstructured extensions can be seen on the left side of the picture.

DOI: 10.7287/peerj.preprints.26997v1/supp-6

Script used for modeling of Aim23p binding to 30S ribosomal subunit

DOI: 10.7287/peerj.preprints.26997v1/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Sergey Levitskii conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, approved the final draft.

Ksenia Derbikova performed the experiments, analyzed the data, prepared figures and/or tables, approved the final draft.

Andrey V Golovin performed the experiments, analyzed the data, approved the final draft.

Anton Kuzmenko conceived and designed the experiments, performed the experiments, analyzed the data, approved the final draft.

Maria V Baleva performed the experiments, approved the final draft.

Ivan Chicherin performed the experiments, approved the final draft.

Igor A Krasheninnikov analyzed the data, prepared figures and/or tables, approved the final draft.

Piotr Kamenski conceived and designed the experiments, analyzed the data, prepared figures and/or tables, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The raw data are provided in the supplementary tables 1 (all OD measurements for Fig.1) and 2-4 (all OD measurements for Fig.2), as well as supplementary figures 1 (initial scan of Western-blot results on Fig. 2D) and 2 (initial result of the molecular modeling). The script used for molecular modeling is also presented (as a html-file).

Funding

This work was supported by the Russian Science Foundation (Grant 17-14-01005 for experimental work and Grant 14-50-00029 for molecular modeling). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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