OC kappa increases in the levels of glucose, trehalose, TOR-P and transcripts encoding proteins involved in photosynthesis, and basal and secondary metabolisms in Eucalyptus globulus
- Published
- Accepted
- Subject Areas
- Agricultural Science, Biochemistry, Biotechnology, Cell Biology, Plant Science
- Keywords
- Eucalyptus globulus, basal metabolism, growth, glucose, TOR kinase, OC kappa, secondary metabolism, trehalose
- Copyright
- © 2018 Saucedo et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. OC kappa increases in the levels of glucose, trehalose, TOR-P and transcripts encoding proteins involved in photosynthesis, and basal and secondary metabolisms in Eucalyptus globulus . PeerJ Preprints 6:e26500v1 https://doi.org/10.7287/peerj.preprints.26500v1
Abstract
Oligo-carrageenan (OC) kappa increases net photosynthesis, and basal and secondary metabolism enzyme activities in Eucalyptus globulus trees. Here, trees were sprayed on leaves with water (control) or with OC kappa 1 mg ml-1, once a week, four times in total, and cultivated for 17 additional weeks (21 weeks in total). Height, level of glucose, trehalose, TOR phosphorylated in Ser2448 (TOR-P) and transcripts encoding TOR and S6 kinase (S6K) as well as the level of transcripts encoding proteins and enzymes involved in glucose accumulation, photosynthesis, C, N and S assimilation, and synthesis of phenylpropanoid compounds (PPCs) and terpenes were determined. Treated trees showed an increase in height of 105% compared to controls at week 21. Treated trees showed an increase in glucose and trehalose level having an oscillatory pattern with maximal levels for glucose at week 1, 9-11 and 17-19, and for trehalose at weeks 1-3, 5, 8-9, 12, 15-16 and 18-21. TOR-P showed increases from week 1 until the end of the experiment with peaks at weeks 2, 6, 12 and 16. The level of tor transcripts showed peaks at weeks 3, 6, 10-11 and 13 whereas the level of s6k transcripts remained unchanged. In addition, transcripts encoding proteins involved in photosynthesis, and enzymes involved in glucose accumulation, C, N and S assimilation, and synthesis of secondary metabolites showed an oscillatory pattern with increases mainly at weeks 3-4, 5-6, 10-11, and in some cases at weeks 13-14 and 16-18. Thus, the increases in trehalose levels better correlate with increases in TOR-P and transcript levels. Therefore, OC kappa induced an increase in the levels of glucose, trehalose, TOR-P and expression of genes involved in photosynthesis, and basal and secondary metabolism which may explain, at least in part, the increase in growth and defense responses in E. globulus trees.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Western blot raw film images
Film images of Western blot using anti-TRP-P and anti-RbcL
Primer sequences for Eucalyptus qPCR
Primer sequences for qPCR of metabolic genes of Eucalyptus globulus, detailing annealing temperature and product size.