Spatiotemporal succession of ammonia-oxidizing archaea abundance and composition in a created riparian wetland
Author and article information
Abstract
Cascaded performance and compartments of constructed wetlands may harbor the internal eco-environmental gradients and further differentiate the niche and community structure of ammonia-oxidizing archaea (AOA). Here, we used quantitative PCR and 454 pyrosequencing of AOA amoA gene to investigate the abundance, diversity, composition, and driving factors of sedimentary AOA community in a created riparian wetland---Shijiuyang constructed wetland (SJY-CW) in China. Results showed that the heterogeneous wetland with serial-connected combinations of pretreatment pond, plant-bed/ditch system, and post-treatment pond as well as the third through fifth year succession (2011--2013, corresponding to the third through fifth year of actual operation after the initial two-year trial operation) had exerted significant spatiotemporal niche selection effects on AOA abundance and composition. AOA abundance in the pre- and post-treatment ponds was almost invariant in 2011--2012 and decreased in 2013, while the abundance in the plant-bed/ditch system decreased gradually with the succession of SJY-CW. The AOA abundance ended up with one order of magnitude lower and tended to be consistent in the whole wetland. In contrast to abundance, the temporal niche differentiation of AOA diversity was insignificant. Nonetheless, a rough decrease trend was detected for the average community richness index Chao1 and diversity index Shannon H' in 2011--2013. Community structure analysis showed that Nitrososphaera was dominant genus, while Nitrosopumilus cluster and Nitrososphaera sister cluster increased with the succession of SJY-CW. Hierarchical clustering analysis and redundancy analysis both verified the horizontal shifts of AOA communities and these shifts inclined to occur in the most heterogeneous plant-bed/ditch system. Operational duration of the wetland which comprehensively reflected the wetland hydrology, water quality, and associated functions became the key factor driving the AOA abundance and community shift in SJY-CW sediments. In a nutshell, with the third through fifth year succession of wetland, the sedimentary ammonia-oxidizing archaea tended to be characterized by temporally decreased and spatially consistent abundance, while spatially variable and temporally decreased richness and diversity; regarding the AOA community structure, temporally from local species and relatively uniform distribution to mixing with allochthonous species and highly diversified distribution, while spatially occurring significant horizontal shifts of AOA amoA genes. These results showed the considerable if not significant niche selection effect of AOA abundance, diversity, and community structure with the operation of SJY-CW, and this will have some implications on the microbial ecology of archaeal ammonia-oxidization in a man-made wetland ecosystem.
Cite this as
2016. Spatiotemporal succession of ammonia-oxidizing archaea abundance and composition in a created riparian wetland. PeerJ Preprints 4:e2629v1 https://doi.org/10.7287/peerj.preprints.2629v1Author comment
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Supplemental Information
LaTeX source files and figures
LaTeX source files and figures
Supplemental tables and figures
Supplemental tables and figures
Raw data for DNA sequencing 454 Reads
Raw data for DNA sequencing 454 Reads
Raw data for physicochemical basics, biodiversity, (sub)clusters, and UIA calculation in the AOA succession study
Raw data for physicochemical basics, biodiversity, (sub)clusters, and UIA calculation in the AOA succession study
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Yu Su performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Guibing Zhu conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Baoling Wang performed the experiments, conducted field sampling..
Yu Wang performed the experiments, conducted field sampling..
Linjie Zhuang analyzed the data.
Weidong Wang conceived and designed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper, conducted field sampling, constructed the paper..
Field Study Permissions
The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):
Field experiments were approved by the Jiaxing Science and Technology Bureau (project number: 2011AZ2019, 2015AY23008). Our research work did not involve capture or any direct manipulation or disturbance of animals. We collected samples of sediments and water for molecular and physicochemical analyses. We obtained access to the wetland under the permission of the Jiaxing Water Conservancy Investment Company Ltd. (Jiaxing, Zhejiang, China), which is responsible for the operation and management of the wetland.
DNA Deposition
The following information was supplied regarding the deposition of DNA sequences:
Sequences were submitted to the Sequence Read Archive (SRA) at the National Center for Biotechnology Information (NCBI) under the accession number range from SRR4244883 to SRR4244897 in a single BioProject SRP089950.
Data Deposition
The following information was supplied regarding data availability:
The raw data has been supplied as a supplementary file.
Funding
This research was jointly funded of equal importance by the National Natural Science Foundation of China (51278487) and Major National Water Pollution Control and Management Project of China (2012ZX07403-003-03), as well as Jiaxing Science and Technology Project (2015AY23008). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
