Figure S1. Pictures of sorted specimens
Pictures of the specimens sorted into small, medium and large individuals. Also provides information on how S, M and L tissue was pooled to generate the proportionally sorted (So) and unsorted (Un) samples.
Figure S2. Flowchart detailing laboratory processing
Overview of the steps carried out for sample sorting and processing in the laboratory.
Figure S3. DNA extraction protocol
Shows the step where the digested buffers of S, M and L were pooled to generate unsorted (Un) and sorted (So) samples.
Figure S4. Sequencing depth and sequences discarded in bioinformatic processing
Barplot showing the number of total reads and proportion of sequences discarded in subsequent bioinformatic processing steps for all samples.
Figure S5. Flowchart detailing the bioinformatic pipeline
Figure giving an overview of the metabarcoding pipeline applied to this dataset.
Figure S6. Reproducibility between HiSeq lanes
Comparison of relative OTUs abundances between both HiSeq lanes.
Figure S7. Plot of OTU table
Visualisation of taxa detected within S, M, L, Un, So DNA extractions, with 4 different primer combinations. Data is also compared to morphological identifications and number of specimens of each morphologically identified taxon.
Figure S8. Database completeness
Plot showing the percent match of each OTU to the reference database, under consideration of read abundance.
Figure S9. Taxa identification with metabarcoding and morphology
Comparison of number of taxa identified with morphology and DNA metabarcoding on different taxonomic resolutions.
Figure S10. Taxa detection in sorted and unsorted samples
Comparison of the amount of diversity and taxa detected in sorted samples (So) and unsorted samples (Un).
Table S1. Used identifiaction keys
Used litrature for taxa identification
Table S2. OTU table
Detailed OTU table giving the number of reads for each sample, including assigned taxonomy and OTU sequence.
Table S3. Morphologically identified taxa
Table giving an overview of morphologically identified taxa and abundance of specimens in S, M and L for both sample locations.