Transcriptomic response of maize primary roots to low temperatures at seedling emergence
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Abstract
Background. Maize (Zea mays) is C 4 tropical cereal and its adaptation to temperate climates can be problematic due to low soil temperatures at early stages of establishment. Methods. We report on a physiological and transcriptomic experiment on twelve maize varieties from a chilling condition adapted gene pool which identified four genotypes with significant contrasting chilling tolerance.These four varieties were subject to microarray analysis to identify up and down regulated genes under chilling conditions. Results. Stress induced by low temperature in the varieties Picker,PR39B29, Fergus and Codisco was reflected only in the expression profiles of the varieties Picker and PR39B29. No significant changes in expression were observed in Fergus and Codisco upon chilling stress. The overall number of genes up and down regulated in the two chilling tolerant varieties amounted to69. These two varieties exhibited two different trancriptomic patterns in which only four genes were shared, although not all with the same degree of regulation. Overall the expression pattern was similar between the two chilling tolerant varieties, indicating a common response to chilling stress. Discussion. Varieties with an enhanced root/shoot growth ratio under low temperature were more tolerant which could be an early and inexpensive measure for germ plasm screening. We have identified novel cold inducible genes in an already adapted maize breeding gene pool. This illustrates that further varietal selection for enhanced chilling tolerance is possible in an already preselected gene pool.
Cite this as
2016. Transcriptomic response of maize primary roots to low temperatures at seedling emergence. PeerJ Preprints 4:e2372v1 https://doi.org/10.7287/peerj.preprints.2372v1note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
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This is a submission to PeerJ for review.
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Supplemental Information
Supplemental Materials Table 1
Maize varieties included in this study. Maize varieties differed in the type of kernel and maturity group. Some varieties were included in the Irish Recommended List 2008 for showing high performance under Irish climate conditions. (*varieties included in the Irish Recommended List 2008 suitable for growing in the open/without plastic; **varieties included in the Irish Recommended List 2008 suitable for growing covered/with plastic; varieties marked in bold were included in the microarray study).
Supplemental Materials Table 2
Housekeeping gene information: Name of gene, accession number and bibliographic reference.
Supplemental Materials Table 3
Differential expressed genes in cultivar PR39B29
Supplemental Materials Table 4
Differential expressed genes in cultivar Picker
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Mauro Di Fenza performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Bridget Hogg performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.
Jim Grant analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Susanne Barth conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.
Microarray Data Deposition
The following information was supplied regarding the deposition of microarray data:
GEO GSE72508 - http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72508
Data Deposition
The following information was supplied regarding data availability:
All gene expression data have been submitted as part of the GEO submission.
Funding
This research was support by the Irish Department of Agriculture, Food and the Marine (DAFM) Stimulus Research grant RSF 07 501 to SB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
