Targeted manipulation of neuronal activity in behaving adult flies
A peer-reviewed article of this Preprint also exists.
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Abstract
The ability to control the activity of specific neurons in freely behaving animals provides an effective way to probe the contributions of neural circuits to behavior. Wide interest in studying principles of neural circuit function using the fruit fly Drosophila melanogaster has fueled the construction of an extensive transgenic toolkit for performing such neural manipulations. Here we describe approaches for using these tools to manipulate the activity of specific neurons and assess how those manipulations impact the behavior of flies. We also describe methods for examining connectivity among multiple neurons that together form a neural circuit controlling a specific behavior. This work provides a resource for researchers interested in examining how neurons and neural circuits contribute to the rich repertoire of behaviors performed by flies.
Cite this as
2016. Targeted manipulation of neuronal activity in behaving adult flies. PeerJ Preprints 4:e2354v2 https://doi.org/10.7287/peerj.preprints.2354v2note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
Author comment
In version two, an error in Figure 5b was corrected. The chromosome was incorrectly labeled as two rather than three. An additional author affiliation was also added. Finally, the top margin was dropped on all pages.
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Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Stefanie Hampel conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.
Andrew Michael Seeds conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Data Deposition
The following information was supplied regarding data availability:
There is no raw data associated with this work.
Funding
This work was supported by the Howard Hughes Medical Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
