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Supplemental Information

Time series measurements of metabolites

This excel file contains the concentrations of the metabolites on different time points and under different conditions.

DOI: 10.7287/peerj.preprints.2077v2/supp-1

A software program for solving the kinetic rates given the time series of concentrations

This Mathematica notebook solves the time-varying enzyme concentrations, given the time-series of concentrations and a hypothetical metabolic network.

DOI: 10.7287/peerj.preprints.2077v2/supp-2

microarray measurements of glycosyl transferases

This dataset was used to compare the estimated enzymatic rates from the mathematical model to the actual expression rates of the enzymes that are potentially involved in the reactions.

DOI: 10.7287/peerj.preprints.2077v2/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Laura Astola analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Hans Stigter analyzed the data, reviewed drafts of the paper.

Maria Victoria Gomez Roldan performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Fred van Eeuwijk conceived and designed the experiments, reviewed drafts of the paper.

Robert D Hall conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Marian Groenenboom analyzed the data, reviewed drafts of the paper.

Jaap J. Molenaar conceived and designed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a Supplemental Dataset

Funding

The Netherlands Consortium for Systems Biology funded this research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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