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Supplemental Information

SI Text S1

Structure analysis and AMOVA on SNP data for Gonioctena quinquepunctata.

DOI: 10.7287/peerj.preprints.1899v1/supp-1

SI Text S2

Calculations of conversion of cyanogenic glycoside contents for dry and fresh weight leaves.

DOI: 10.7287/peerj.preprints.1899v1/supp-2

Table S1

Full data on identities and numbers of herbivores collected on each individual Prunus serotina and Prunus padus in National Park Zuid-Kennemerland.

DOI: 10.7287/peerj.preprints.1899v1/supp-3

Table S2

Information on insect herbivore damage in historical collection specimens from the National Herbarium, Leiden, for Prunus serotina and Prunus padus.

DOI: 10.7287/peerj.preprints.1899v1/supp-4

Table S3

Full data on NMR analysis of Prunus leaves.

DOI: 10.7287/peerj.preprints.1899v1/supp-5

Table S4

SNP genotype data on Gonioctena quinquepunctata.

DOI: 10.7287/peerj.preprints.1899v1/supp-6

Table S5

Oligonucleotides used for the SNP-analysis of Gonioctena quinquepunctata.

DOI: 10.7287/peerj.preprints.1899v1/supp-7

Table S6

SNP Loci that showed indications of genetic differentiation between both host plants in the leaf beetle Gonioctena quinquepunctata.

DOI: 10.7287/peerj.preprints.1899v1/supp-8

Table S7

ANOVA and GLM results for the Gonioctena quinquepunctata host preference tests.

DOI: 10.7287/peerj.preprints.1899v1/supp-9

Table S8

Explanatory variables and response variables for the test of parasitzation of caterpillars on P. serotina and P. padus.

DOI: 10.7287/peerj.preprints.1899v1/supp-10

Additional Information

Competing Interests

Marco Flohil is an employee of ServiceXS, a company providing DNA services such as reported in this paper.

Author Contributions

Menno Schilthuizen conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Lúcia P Santos Pimenta performed the experiments, analyzed the data.

Youri Lammers performed the experiments, analyzed the data.

Peter J Steenbergen performed the experiments.

Marco Flohil performed the experiments.

Nils G.P. Beveridge performed the experiments.

Pieter T van Duijn performed the experiments.

Marjolein M Meulblok performed the experiments.

Nils Sosef performed the experiments.

Robin van de Ven performed the experiments.

Ralf Werring performed the experiments.

Kevin K Beentjes performed the experiments.

Kim Meijer conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables.

Rutger A Vos conceived and designed the experiments, analyzed the data.

Klaas Vrieling analyzed the data.

Barbara Gravendeel conceived and designed the experiments.

Young Choi conceived and designed the experiments.

Robert Verpoorte conceived and designed the experiments.

Chris Smit conceived and designed the experiments.

Leo W Beukeboom conceived and designed the experiments.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Natuurmonumenten (Ruud Luntz) permitted us to work in Nationaal Park Zuid-Kennemerland under permit No. 19 of 2008. Dunea (Harrie van der Hagen) permitted us to work in Meijendel by permission 25/2/2013.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The data were deposited in the NCBI short read archive under BioProject accession code: PRJNA277307.

Data Deposition

The following information was supplied regarding data availability:

Supporting Information (uploaded separately)

Table S1. Full data on identities and numbers of herbivores collected on each individual Prunus serotina and Prunus padus in National Park Zuid-Kennemerland.

Table S2. Information on insect herbivore damage in historical collection specimens from the National Herbarium, Leiden, for Prunus serotina and Prunus padus.

Table S3. Full data on NMR analysis of Prunus leaves.

Table S4. SNP genotype data on Gonioctena quinquepunctata.

Table S5. Oligonucleotides used for the SNP-analysis of Gonioctena quinquepunctata.

Table S6. SNP Loci that showed indications of genetic differentiation between both host plants in the leaf beetle Gonioctena quinquepunctata.

Table S7. ANOVA and GLM results for the Gonioctena quinquepunctata host preference tests.

Table S8. Explanatory variables and response variables for the test of parasitzation of caterpillars on P. serotina and P. padus.

SI Text S1. Structure analysis and AMOVA on SNP data for Gonioctena quinquepunctata.

SI Text S2. Calculations of conversion of cyanogenic glycoside contents for dry and fresh weight leaves.

Funding

This study was financially supported by grants from the Uyttenboogaart-Eliasen Foundation and the Team for Invasive Exotics of the former Netherlands Ministry for Agriculture, Nature, and Food Quality. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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