Altered ADAR1 in mice affected by social isolation stress-induced cognitive deficits
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Abstract
Adenosine deaminase acting on RNA (ADAR) activity increases in response to inflammation. Social isolation stress is related to neuroinflammation; however, it remains unclear whether ADAR1 is altered in response to social isolation stress-induced cognitive deficits. To investigate our hypothesis that ADAR1 displayed patterns of change in response to social isolation stress, we addressed this issue systemically by isolating Kunming mice for 2, 4 and 8 weeks individually since postnatal 21 days to set up isolation mouse model. Furthermore, we arranged re-socialization group to evaluate the alterations of ADAR1 in the cognitive deficits recovery. The results of behavior tests showed that social isolation stress resulted in cognitive dysfunction, which was recovered by re-socialization in re-gregarious rearing group. Furthermore, the immunohistochemistry and western blot results displayed that both the immunoreactivity and protein expression of ADAR1 in the hippocampus and frontal cortex increased obviously as compared to the same age mice without isolation. The above abnormal alterations of ADAR1 were recovered by re-socialization in re-gregarious rearing group. Our study supports the hypothesis that ADAR1 is altered in mice affected by social isolation stress-induced cognitive deficits.
Cite this as
2016. Altered ADAR1 in mice affected by social isolation stress-induced cognitive deficits. PeerJ Preprints 4:e1870v1 https://doi.org/10.7287/peerj.preprints.1870v1note This preprint is not peer-reviewed. You may wish to reference the subsequent peer-reviewed version of this article.
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This is a preprint submission to PeerJ PrePrints.
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Supplemental Information
Figure 1: Experimental design for groups divided
Figure 2: Spontaneous alternation times in Tmaze in Kunming mice
Figure 3: Immunohistochemical analysis of ADAR1 positive cells in the brain of Kunming mice
Figure 4: The results of Western blot in the hippocampus and frontal cortex of Kunming mice
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Wei Chen conceived and designed the experiments, wrote the paper, prepared figures and/or tables.
Dong An conceived and designed the experiments, prepared figures and/or tables.
Hong Xu performed the experiments.
Xiaoxin Cheng performed the experiments.
Shiwei Wang analyzed the data.
Weizhi Yu performed the experiments.
Deqin Yu performed the experiments.
Dan Zhao performed the experiments, prepared figures and/or tables.
Yiping Sun contributed reagents/materials/analysis tools.
Wuguo Deng contributed reagents/materials/analysis tools.
Yiyuan Tang contributed reagents/materials/analysis tools, advice the manuscript.
Shengming Yin conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.
Animal Ethics
The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):
The Tab of Animal Experimental Ethical Inspection - Number: L20140021
Data Deposition
The following information was supplied regarding data availability:
we will provide the raw data.
Funding
This work was supported by grants from the National Natural Science Foundation of China(31201724). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
