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Detection of a Yersinia pestis gene homologue in rodent samples

, , , , , ,
1 Veterinary Medicine and Science, University of Nottingham, Loughborough, Leicestershire, United Kingdom
2 Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany
3 Department of Translational Genetics, Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus
4 School of Veterinary Medicine and Science, University of Nottingham, Loughborough, Leicestershire, United Kingdom
DOI
10.7287/peerj.preprints.1866v1
Published
Accepted
Subject Areas
Genetics, Microbiology, Molecular Biology
Keywords
Yersinia pestis, rodents, screening, microarray
Copyright
© 2016 Giles et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Giles TA, Greenwood AD, Tsangaras K, Barrow PA, Hannant D, Abu-Median A, Yon L. 2016. Detection of a Yersinia pestis gene homologue in rodent samples. PeerJ Preprints 4:e1866v1

Abstract

A homologue to a widely used genetic marker, pla, for Yersinia pestis has been identified in tissue samples of two species of rat (Rattus rattus and Rattus norvegicus) and of mice (Mus musculus and Apodemus sylvaticus) using a microarray based platform to screen for zoonotic pathogens of interest. Samples were from urban locations in the UK (Liverpool) and Canada (Vancouver). The results indicate the presence of an unknown bacterium that shares a homologue with Yersinia pestis.

Author Comment

This is a submission to PeerJ for review.

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Timothy A Giles conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Alex D Greenwood conceived and designed the experiments, reviewed drafts of the paper.

Kyriakos Tsangaras conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Paul A Barrow reviewed drafts of the paper.

Duncan Hannant reviewed drafts of the paper.

Abu-Bakr Abu-Median conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Lisa Yon conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

I have a letter of approval from the School of Veterinary Medicine and Science which I can send to you, further approval was not required, because all of the samples used in this project were not collected either by us, or for the purpose of this project. The samples were simply tissues that remained from other projects being conducted by our collaborators.

Microarray Data Deposition

The following information was supplied regarding the deposition of microarray data:

GSE77765

Data Deposition

The following information was supplied regarding data availability:

The raw data is available from GEO GSE77765

Funding

This research has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 222633. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Detection of a Yersinia pestis gene homologue in rodent samples
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