Transcription factor binding site prediction of the procine KL intron 1 containing MARC0022311 (KL g.1474 A>G)
Quadrilateral frame indicated the substitutions and extra binding site of OCT-1. (A) Predicted by BIOBASE online software. (B) Predicted by TFserach online software.
Genotyping results of MARC0022311
(A) PK cells. (B) ST cells. MARC0022311 was marked in gray backgound.
OCT-1 binding sites in the porcine KL intron 1
(A) Frequency distribution of the predicted OCT-1 binding sites. X-axis indicated the length of the porcine KL intron 1 in bp. Y-axis was the frequency of the predicted OCT-1 binding sites. (B) ChIP analysis of three candidate OCT-1 binding sites (1395 bp to 1525 bp, 14322 bp to 14436 bp, 30970 bp to 31141 bp) in KL intron 1 in PK cells. Primers used for ChIP-PCR was shown in Table 1. Input and R were positive control, while IgG was the negative control.