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Supplemental Information

Transcription factor binding site prediction of the procine KL intron 1 containing MARC0022311 (KL g.1474 A>G)

Quadrilateral frame indicated the substitutions and extra binding site of OCT-1. (A) Predicted by BIOBASE online software. (B) Predicted by TFserach online software.

DOI: 10.7287/peerj.preprints.1800v1/supp-1

Genotyping results of MARC0022311

(A) PK cells. (B) ST cells. MARC0022311 was marked in gray backgound.

DOI: 10.7287/peerj.preprints.1800v1/supp-2

OCT-1 binding sites in the porcine KL intron 1

(A) Frequency distribution of the predicted OCT-1 binding sites. X-axis indicated the length of the porcine KL intron 1 in bp. Y-axis was the frequency of the predicted OCT-1 binding sites. (B) ChIP analysis of three candidate OCT-1 binding sites (1395 bp to 1525 bp, 14322 bp to 14436 bp, 30970 bp to 31141 bp) in KL intron 1 in PK cells. Primers used for ChIP-PCR was shown in Table 1. Input and R were positive control, while IgG was the negative control.

DOI: 10.7287/peerj.preprints.1800v1/supp-3

Raw SNP genotyping results

DOI: 10.7287/peerj.preprints.1800v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Yan Li performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Lei Wang performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jiawei Zhou analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Fenge Li conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All animal procedures were performed according to protocols approved by the Biological Studies Animal Care and Use Committee of Hubei Province, PR China. Sample collection was approved by the ethics committee of Huazhong Agricultural University (No.30700571 for this study).

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a Supplemental Dataset.

Funding

This work was supported financially by Key Projects in National Science R&T Program (2015BAD03B02, 2014BAD20B01), Hubei Science R&T Program (2014BBB008, 2014BBA194), and Fundamental Research Funds for the Central Universities. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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