SimpleDSFviewer: a tool to analyse and view differential scanning fluorimetry data for characterising protein thermal stability and interactions
Biochemistry, University of Liverpool, Liverpool, United Kingdom
- Published
- Accepted
- Subject Areas
- Biochemistry
- Keywords
- differential scanning fluorimetry (DSF), protein melting temperature, protein melting curve, data analysis, protein interactions, screening, tool compound, small molecule inhibitor, software
- Copyright
- © 2015 Sun et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2015. SimpleDSFviewer: a tool to analyse and view differential scanning fluorimetry data for characterising protein thermal stability and interactions. PeerJ PrePrints 3:e1555v1 https://doi.org/10.7287/peerj.preprints.1555v1
Abstract
Differential scanning fluorimetry (DSF) is used widely as a thermal shift assay to study protein stability and protein-ligand interactions. The benefit of DSF is that it is simple, cheap and can generate melting curves in 96-well plates providing good throughput. However, data analysis remains a challenge, and requires different methods to optimise and analyse the collected raw data. Here, the program SimpleDSFviewer is introduced to help view and analyse DSF data in an efficient way and with a user-friendly interface. The data analysis, optimisation and view methods provided by the program are described, using sample melting curves of fibroblast growth factors.
Author Comment
This is a preprint submission to PeerJ Preprints.