SimpleDSFviewer: a tool to analyse and view differential scanning fluorimetry data for characterising protein thermal stability and interactions
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Abstract
Differential scanning fluorimetry (DSF) is used widely as a thermal shift assay to study protein stability and protein-ligand interactions. The benefit of DSF is that it is simple, cheap and can generate melting curves in 96-well plates providing good throughput. However, data analysis remains a challenge, and requires different methods to optimise and analyse the collected raw data. Here, the program SimpleDSFviewer is introduced to help view and analyse DSF data in an efficient way and with a user-friendly interface. The data analysis, optimisation and view methods provided by the program are described, using sample melting curves of fibroblast growth factors.
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2015. SimpleDSFviewer: a tool to analyse and view differential scanning fluorimetry data for characterising protein thermal stability and interactions. PeerJ PrePrints 3:e1555v1 https://doi.org/10.7287/peerj.preprints.1555v1Author comment
This is a preprint submission to PeerJ Preprints.
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Competing Interests
The authors declare they have no competing interests.
Author Contributions
Changye Sun conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.
Yong Li performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.
Edwin A Yates conceived and designed the experiments, analyzed the data, reviewed drafts of the paper.
David G Fernig conceived and designed the experiments, reviewed drafts of the paper.
Data Deposition
The following information was supplied regarding data availability:
GitHub
https://github.com/hscsun/SimpleDSFviewer.git
Funding
This work was funded by North West Cancer Research and the Cancer and Polio Research Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
