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Mitochondrial phylogenomics and genetic population structure of anchovies (Engraulis encrasicolus) along the Moroccan coast using sequence analysis of the mitochondrial DNA cytochrome b

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1 Département de Biologie, Faculté des Sciences Rabat, Mohammed V University Rabat, Rabat, Rabat, Morocco
2 Département de Biologie, Faculté des Sciences Rabat, Mohammed V University Rabat, Rabat, Rabat, Morocco
3 Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan
4 Département des Ressources Halieutiques, Institut National de Recherche Halieutique (INRH), Casablanca, Grand Casablanca, Maroc
DOI
10.7287/peerj.preprints.1543v1
Published
Accepted
Subject Areas
Aquaculture, Fisheries and Fish Science, Biodiversity, Ecology, Genetics, Marine Biology
Keywords
European anchovy (Engraulis encrasicolus), Morocco, mitochondrial cytochrome b, phylogeography, population structure
Copyright
© 2015 Chahdi Ouazzani et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
Cite this article
Chahdi Ouazzani K, Benazzou T, Tojo N, Chlaida M. 2015. Mitochondrial phylogenomics and genetic population structure of anchovies (Engraulis encrasicolus) along the Moroccan coast using sequence analysis of the mitochondrial DNA cytochrome b. PeerJ PrePrints 3:e1543v1

Abstract

A fragment of 680 bp of mitochondrial cytochrome b locus of European anchovies, Engraulis encrasicolus, was sequenced for 138 individuals collected from three Moroccan Atlantic areas and from Moroccan Alboran Sea. These samples were surveyed for diversity and differentiation with a range of summary statistics. The results showed that the most dominant clade in Moroccan anchovy is Clade A with a percentage ranging from 89% in Alboran Sea to 91% - 95 % in the Moroccan Atlantic coast. Overall, there was a significant genetic differentiation among the 4 Moroccan anchovy zones (Φst = 0.01283; p=0.03910). Pairwise Φst among populations and multidimensional scaling revealed a high homogeneity among Atlantic populations but some heterogeneity between Alboran population and Atlantic populations, mainly between the populations from Central Atlantic of Morocco with a significant difference.

Author Comment

This is a preprint submission to PeerJ.

Supplemental Information

sequence submitted in the GenBank

DOI: 10.7287/peerj.preprints.1543v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Khalil Chahdi Ouazzani conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Touria Benazzou conceived and designed the experiments, reviewed drafts of the paper.

Naoki Tojo reviewed drafts of the paper.

Malika Chlaida conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

1.Institut National de Recherche Halieutique "INRH"

2.Sampling and analysis techniques used in this work are consistent with the Moroccan legislation Article 14, Section 3, Bill 122.22 and the Cartagena Protocol on Biosafety to the Convention on Biological Diversity promulgated by the royal decree "1.09.123".

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Sequences were submitted in the GenBank database with the following accession numbers: (BankIt1837228: KT246173 - KT246289)

Data Deposition

The following information was supplied regarding data availability:

the raw data is submitted in the GenBank but not yet published, but we give for reviewers our dataset as a Supplemental Files

Funding

This work was supported by the INRH, Faculté des Sciences de Rabat (Mohammed V University, Rabat) and CNRS (University of Montpellier, France) and partially by IMPM project which is a collaboration between Japanese International Cooperation Agency and Morocco. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Mitochondrial phylogenomics and genetic population structure of anchovies (Engraulis encrasicolus) along the Moroccan coast using sequence analysis of the mitochondrial DNA cytochrome b
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