Platelet factor 4 inhibits human hair follicle growth and promotes androgen receptor expression in human dermal papilla cells

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Biochemistry, Biophysics and Molecular Biology

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Introduction

Material and Methods

Human hair follicle isolation and culture

hDPCs isolation and culture

RNA collection and qPCR

Western blot assays

Immunofluorescence

ELISA

Statistical analysis

Results

PF4 suppresses hair growth in cultured human HFs

PF4 suppresses human dermal papilla cell proliferation

PF4 increases the expression of androgen receptor in hDPCs

PF4 declines the hair growth-promoting properties of hDPCs

Discussion

Conclusions

Supplemental Information

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Ke Sha conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Mengting Chen and San Xu performed the experiments, prepared figures and/or tables, and approved the final draft.

Fangfen Liu and Qinqin Peng performed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Ben Wang, Yiya Zhang and Hongfu Xie analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Ji Li conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Zhili Deng conceived and designed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Institutional Review Board of Xiangya hospital approved this research (IRB NO. 201611609).

Data Availability

The following information was supplied regarding data availability:

The raw data is available at Figshare: Deng, Zhili (2020): Sha-2020-Rawdata.rar. figshare. Figure. https://doi.org/10.6084/m9.figshare.11828610.v1.

Funding

This work was supported by the National Natural Science Foundation of China (No. 81773351, No. 91749114, No. 81874251, No. 81703149, No. 81602789). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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