An immunoinformatics approach for the design of a multi-epitope subunit vaccine for urogenital schistosomiasis

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Methodology

Retrieval of Schistosoma haematobium HSP sequence for vaccine construction

Prediction of cytotoxic T-lymphocytes and helper T-lymphocytes epitope and immunogenicity

Construction of multi-epitope subunit vaccine

B cell epitopes prediction for S. haematobium proteins

Prediction of antigenicity, allergenicity and physiochemical parameter of vaccine construct

Prediction, refinement and validation of the tertiary structure of vaccine construct

Disulfide engineering for vaccine stability

Codon adaptation and in silico cloning

Molecular docking of vaccine construct with TLR4

Results

Retrieved S. haematobium HSP sequences for vaccine construct

Predicted CTL, HTL epitope and immunogenicity

Constructed multi-epitope subunit vaccine

Predicted B cell epitopes for S. haematobium proteins

Predicted antigenicity, allergenicity and physiochemical parameter of the vaccine construct

Predicted, refined and validated tertiary structure of vaccine construct

Disulfide engineering for vaccine stability

Codon adaptation and in silico cloning

Molecular docking of vaccine construct with TLR4

Discussion

Conclusion

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Olugbenga S. Onile conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Adeyinka I. Fadahunsi performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Ameerah A. Adekunle conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Bolaji F. Oyeyemi conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Chiaka I. Anumudu analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The HSP sequences available at NCBI: HSP90 (KGB37337.1), HSP11 (XP_012799478.1), HSP97 (KGB34481.1), 212 HSP75 (KGB38609.1), HSP83 (KGB37622.1), HSP10 (KGB41273.1), HSP60 (KGB40455.1).

Additional data used in this study is available at the RCSB-Protein Data Bank: search term 4G8A.

Funding

The authors received no funding for this work.

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