Effects of Trichophyton mentagrophytes infection on the immune response of rabbits

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials & Methods

Experimental animals

Fungal challenge on skin

Isolation and cultivation of fungi from the challenge site

Fungal observation under microscope

DNA extraction and PCR of cerebellar degeneration-related protein 1 (cdr1) gene

Periodic acid-Schiff (PAS) staining of tissue sections of FI group

Blood sampling and serum isolation

Antibody and cytokine assay

RNA isolation from blood samples and fluorescence-based quantitative PCR

Statistical analysis

Results

Symptoms and pathogen identifications

Blood cell analysis

Antibody and cytokine

Results of RT-PCR

Discussion

Conclusions

Supplemental Information

Blood test raw data

DOI: 10.7717/peerj.7632/supp-3

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Chenwen Xiao conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Guolian Bao contributed reagents/materials/analysis tools, approved the final draft.

Qiang Wei analyzed the data, approved the final draft.

Yan Liu contributed reagents/materials/analysis tools, approved the final draft.

Jiaoyu Wang symptoms and Pathogen Identifications on Trichophyton mentagrophytes challenge.

Quanan Ji prepared figures and/or tables, approved the final draft.

YeE Huang prepared figures and/or tables, approved the final draft, contribute for english reading.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All animal experiments in this study were approved by the Animal Ethics Committee of the Zhejiang Academy of Agricultural Science (No: 1905).

Data Availability

The following information was supplied regarding data availability:

Data is deposited in the Sequence Read Archive: GSE80604, SRA: SRP073785.

Funding

This animal challenge model experiment and the design of the study and collection in this study was funded by the National Rabbit Industry Project of China (nycytx-44-3-2) and the RT-PCR, ELISA experiment and analysis and interpretation of data and in writing the manuscript were funded by the National Natural Science Foundation (31402241) and Basic Public Welfare Research Projects in Zhejiang Province (LGN18C180003) and Key research and development plan of Zhejiang Province (No. 2016C02054-10). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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