Conservation of transcriptional elements in the obligate symbiont of the whitefly Bemisia tabaci

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Microbiology

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Introduction

Materials & Methods

Genome resources

Phylogenetic analysis

Recognization of transcription-related genes

MED whitefly heat shock treatment

Functional elements searching in intergenic spacers

Results and Discussion

The divergence and function of Portiera

Genes related with transcription

Heat shock treatment

Putative functional elements in intergenic spacers of Portiera genomes

Conclusions

Supplemental Information

Primers utilized in quantitative PCR

DOI: 10.7717/peerj.7477/supp-1

Sequence similarities (%) between different Portiera species pairs

DOI: 10.7717/peerj.7477/supp-2

No retention of regulatory genes for essential amino acid biosynthesis pathways in Portiera inferred from Escherichia coli

DOI: 10.7717/peerj.7477/supp-3

Calculation of Ka/Ks ratio of genes cspA between orthologous pairs from different Portiera species

DOI: 10.7717/peerj.7477/supp-4

List of 95 intergenic spacers (IGS) orthologs, 51 perfectly aligned IGS (over 20 nucleotides), 37 Shine-Dalgarno sequences and 34 putative small RNAs

DOI: 10.7717/peerj.7477/supp-5

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Dan-Tong Zhu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

Chi Zou and Fei-Xue Ban performed the experiments.

Hua-Ling Wang prepared figures and/or tables, authored or reviewed drafts of the paper.

Xiao-Wei Wang conceived and designed the experiments, contributed reagents/materials/analysis tools.

Yin-Quan Liu conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The sequences of Portiera from whitefly B. tabaci Asia II 3 (previously referred to as ZHJ1 ‘biotype’) (Portiera BT-Z1) and Portiera from whitefly B. tabaci China 1 (previously referred to as ZHJ3 ‘biotype’) (Portiera BT-Z3) are available in GenBank under accession numbers CP016327 and CP016343.

Funding

Financial support for this study was provided by the National Key Research and Development Program (2016YFC1200601), the China Agriculture Research System (CARS-23-D07), National Natural Science Foundation of China (31390421), and the China Postdoctoral Science Foundation (Grant Number 517000-X91502). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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