Membrane potential (Vmem) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation

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RT @iMitbhavsar: My article has been published today in @thePeerJ https://t.co/mRnHBeJeXE #Bioengineering #CellBiology #Statistics
Membrane potential (Vmem) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation https://t.co/DK8Jnrapef @thePeerJ https://t.co/2jnKLzSdWB
RT @iMitbhavsar: My article has been published today in @thePeerJ https://t.co/mRnHBeJeXE #Bioengineering #CellBiology #Statistics
My article has been published today in @thePeerJ https://t.co/mRnHBeJeXE #Bioengineering #CellBiology #Statistics
Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

  1. Raw BF image – DF image = DF corrected BF image

  2. FF image – DF image = DF corrected FF image

  3. DF corrected BF image – DF corrected FF image = Corrected final Image

Results

Cell proliferation and Vmem measurements

Osteogenic differentiation and Vmem measurements

Discussion

Conclusion

Supplemental Information

AT-MSC proliferation and Vmem measurements.

DOI: 10.7717/peerj.6341/supp-1

AT-MSC osteogenic differentiation and Vmem measurements.

DOI: 10.7717/peerj.6341/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Mit Balvantray Bhavsar conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables and assisted in preparing the manuscript for publication.

Gloria Cato performed the experiments, analyzed the data.

Alexander Hauschild prepared figures and/or tables and assisted in preparing the manuscript for publication.

Liudmila Leppik conceived and designed the experiments, contributed reagents/materials/analysis tools, assisted in preparing the manuscript for publication.

Karla Mychellyne Costa Oliveira assisted in preparing the manuscript for publication.

Maria José Eischen-Loges performed the experiments.

John Howard Barker edited and corrected the manuscript.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental Files 1 and 2. Figure 2 shows raw data regarding AT-MSC proliferation and Fig. 3 regarding AT-MSC osteogenic differentiation.

Funding

The work described herein was supported by the Friedrichsheim Foundation (Stiftung Friedrichsheim) in Frankfurt/Main, Germany. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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