Anopheles gambiae densovirus (AgDNV) has negligible effects on adult survival and transcriptome of its mosquito host

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Introduction

Materials and Methods

Cell culture, mosquito infection and rearing conditions

Life-table analysis

RNA extraction and microarrays

qPCR validation of microarray analysis

Results and Discussion

Life table analysis

Microarray analysis

Additional Information and Declarations

Competing Interests

Xiaoxia Ren is an employee of Pharmaceutics International Inc; all research was completed prior to onset of employment.

Author Contributions

Xiaoxia Ren conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

Grant L. Hughes conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Guodong Niu performed the experiments, prepared figures and/or tables.

Yasutsugu Suzuki performed the experiments, reviewed drafts of the paper.

Jason L. Rasgon conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Microarray Data Deposition

The following information was supplied regarding the deposition of microarray data:

Raw Affymetrix data files are available at:

http://rasgonlab.files.wordpress.com/2014/08/renetalrawaffymetrixdata.zip.

Funding

This research was supported by NIH grants R21AI088311, R21AI111175 and R21AI070178 to JLR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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