Venomix: a simple bioinformatic pipeline for identifying and characterizing toxin gene candidates from transcriptomic data

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Bioinformatics and Genomics

Main article text

 

Introduction

Materials and Methods

Data acquisition and transcriptome assembly

Analysis pipeline and execution

Venomix evaluation

Results

Transcriptome assemblies

Pipeline output

Venomix outputs for C. sponsalis

Venomix outputs for E. coloratus

Venomix outputs for T. bicarinatum

Venomix outputs for U. yaschenkoi

Discussion

Supplemental Information

Supplemental Tables

DOI: 10.7717/peerj.5361/supp-1

Venomix outputs for C. sponsalis used to write this manuscript

DOI: 10.7717/peerj.5361/supp-2

Venomix outputs for E. coloratus used to write this manuscript

DOI: 10.7717/peerj.5361/supp-3

Venomix outputs for T. bicarinatum used to write this manuscript

DOI: 10.7717/peerj.5361/supp-4

Venomix outputs for original T. bicarinatum assembly used to write this manuscript

DOI: 10.7717/peerj.5361/supp-5

Venomix outputs for U. yaschenkoi used to write this manuscript

DOI: 10.7717/peerj.5361/supp-6

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Jason Macrander conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Jyothirmayi Panda conceived and designed the experiments, performed the experiments, approved the final draft.

Daniel Janies and Adam M. Reitzel contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Marymegan Daly conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Data Availability

The following information was supplied regarding data availability:

Venomix Pipeline: https://bitbucket.org/JasonMacrander/venomix.

Funding

Support for this project was provided by NSF DEB 1257796 to Marymegan Daly, NSF DEB 1536530 to Marymegan Daly and Jason Macrander, and NSF OCE 1536530 to Adam Reitzel. Jyothirmayi Panda was supported by a research assistantship provided by the University of North Carolina at Charlotte Graduate School under the guidance of Dan Janies. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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