The salivary secretome of the biting midge, Culicoides sonorensis

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Introduction

Materials and Methods

Culicoides saliva collection

SDS polyacrylamide Gel Electrophoresis (PAGE)

MS/MS sequencing

Database construction

Amino acid sequence and conserved domain analysis

Results and Discussion

Culicoides saliva secretome proteins identified

Conserved domains of Culicoides salivary secretome proteins

Culicoides sonorensis maltase

Culicoides sonorensis late trypsin

Culicoides sonorensis Kunitz-type protease inhibitors

Culicoides sonorensis D7 proteins

Conclusions

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Christopher J. Lehiy performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Barbara S. Drolet conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Funding

This work was supported by the U.S. Department of Agriculture, Agricultural Research Service under the National Programs of Veterinary, Medical and Urban Entomology (Project Number 5430 32000 003) and Animal Health (Project Number 5430 3200 006). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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