Effect of oral tryptamines on the gut microbiome of rats—a preliminary study

View article
Microbiology

Main article text

 

Introduction

Materials and Methods

Production of psilocybin and precursors from E. coli

Animals, housing condition, treatment, and fecal collection

16S rDNA sequencing and analysis

Statistical analysis

Results

Effects of psilocybin and norbaeocystin on microbial ecology and diversity

Effects of psilocybin and norbaeocystin on microbial abundance of dominant phyla

Effects of psilocybin and norbaeocystin on microbial abundance of sub-dominant phyla

Discussion

Conclusions

Supplemental Information

Shannon diversity index.

Shannon diversity index was done utilizing QIIME 2 for differentiation of bacteria at the phylum level.

DOI: 10.7717/peerj.17517/supp-1

Relative abundance of microbial phylum.

Relative abundance refers to the percentage of one microbial phylum in relation to the total number of phyla in the community.

DOI: 10.7717/peerj.17517/supp-2

ARRIVE guidelines 2.0.

DOI: 10.7717/peerj.17517/supp-3

Additional Information and Declarations

Competing Interests

J. Andrew Jones is a significant stakeholder at PsyBio Therapeutics. PsyBio Therapeutics has licensed tryptamine biosynthesis-related technology from Miami University. J. Andrew Jones and Matthew S. McMurray are co-inventors on several patent applications related to tryptamine biosynthesis and the impacts of tryptamines on animal behavior. All other authors declare no conflicts of interest.

Author Contributions

Mengyang Xu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Andor J. Kiss conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

J. Andrew Jones conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Matthew S. McMurray conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Haifei Shi conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All procedures were approved by Miami University’s Institutional Animal Care and Use Committee (IACUC Project Number: 1033_2023_Apr).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The raw sequencing data are available at Bioproject: PRJNA1054120.

http://www.ncbi.nlm.nih.gov/bioproject/1054120

Data Availability

The following information was supplied regarding data availability:

The Shannon diversity and phylum of samples are available in the Supplemental Files.

Funding

This work was supported by a sponsored research grant from PsyBio Therapeutics (J. Andrew Jones, Matthew S. McMurray) and is supported by Miami University Faculty Research grant (Haifei Shi). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

2 Citations 1,327 Views 81 Downloads

Your institution may have Open Access funds available for qualifying authors. See if you qualify

Publish for free

Comment on Articles or Preprints and we'll waive your author fee
Learn more

Five new journals in Chemistry

Free to publish • Peer-reviewed • From PeerJ
Find out more