Carbapenemase genes in clinical and environmental isolates of Acinetobacter spp. from Quito, Ecuador

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Microbiology

Main article text

 

Introduction

Materials and Methods

Clinical isolates

River water collection and bacterial identification

Antimicrobial susceptibility test

Molecular identification of carbapenems-resistant genes

Results

Identification of Acinetobacter spp.

Phenotypic antimicrobial susceptibility

Molecular identification of carbapenem-resistance genes

Carbapenem-resistance genes sequences analysis

Discussion

Acinetobacter spp. in clinical and river isolates

Carbapenem-resistant Acinetobacter spp.

Carbapenemases genes in Acinetobacter spp.

Conclusions

Supplemental Information

Clinical and River isolates data.

All samples correspond to Zone 9, which represents the code for Quito. The table details the hospital code, INSPI isolate code, geographical coordinates age, sex, sample type, and hospital unit of the patients to which each sample corresponds. In addition, the table indicated the river data, id code, geographical coordinates, and river name. Acronyms: NA: not available.

DOI: 10.7717/peerj.17199/supp-1

Biochemical results and antibiotic susceptibility profile of Acinetobacter spp.

NA: Not applied, Aba: A. baumannii, Aha: A. haemolyticus, ABC: A. baumannii/calcoaceticus complex, ng/µl: nanograme/microlitre, Y: yes, N: not. Biochemical results are expressed as positive (+) and negative (−). The antibiotic susceptibility profile are expresed as Intermediate (I), Sensistive (S), and Resistance (R).

DOI: 10.7717/peerj.17199/supp-2

Molecular results of Acinetobacter spp. obtained by amplification and sequencing of different genes.

A. baumannii was identified by the amplification of both products, 490 bp and 294 bp, and A. pittii with a product of 194 bp of gyrB gene. The species identification was corroborate by sequencing on partial rpo gene. PCR results are expressed for carbapenen resistance genes as positive (+) and negative (−). The table details the GenBank number acccess of sequences obtained in this study.

DOI: 10.7717/peerj.17199/supp-3

Partial rpoB gene nucleotide sequence matrix.

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Partial rpoB gene protein sequence matrix.

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blaOXA-23 nucleotide sequences matrix.

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blaOXA-24 nucleotide sequences matrix.

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blaOXA-51 nucleotide sequences matrix.

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blaOXA-143 nucleotide sequence.

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OXA-23 amino acid sequences matrix.

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OXA-24 amino acid sequences matrix.

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OXA-51 amino acid sequences matrix.

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OXA-143 amino acid sequence.

DOI: 10.7717/peerj.17199/supp-13

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Nicole Sotomayor performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, water sample collection, and approved the final draft.

José Eduardo Villacis conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Noela Burneo conceived and designed the experiments, performed the experiments, analyzed the data, authored or reviewed drafts of the article, water sample collection, and approved the final draft.

Jorge Reyes conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Sonia Zapata conceived and designed the experiments, authored or reviewed drafts of the article, funding granted by the USFQ necessary for the sequencing of the complete genes blaOXA-51, blaOXA-23, and blaOXA-24, and approved the final draft.

Rosa de los Ángeles Bayas-Rea conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The sequences are available at GenBank: MF594724MF594783, OP796381.1OP796781.1, OP554146.1OP554193.1.

The raw measurements are available in the Supplemental Files.

Funding

This work was supported by the Pontificia Universidad Católica del Ecuador project (No. L13341) and Universidad San Francisco de Quito (COCIBA Grant). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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