Antibiotic susceptibility and resistance genes profiles of Vagococcus salmoninarum in a rainbow trout (Oncorhyncus mykiss, Walbaum) farm

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Microbiology

Main article text

 

Introduction

Materials & Methods

Sample collection

Morphological and biochemical identification of the isolates

Molecular Identification of the Isolates

Phylogenetic tree construction

Antimicrobial susceptibility evaluation of the isolates

Antibiotic resistance genes screening of the isolates

Calculation of multiple antibiotic resistance index (MAR) value

Ethical statement

Results

Morphological and biochemical identification of the isolates

Molecular identification and phylogeny of the isolates

Antibiotic resistance gene profiles of the isolates

Antimicrobial susceptibility of the isolates

Discussion

Conclusions

Supplemental Information

Biochemical characterization of Vagococcus salmoninarum isolates

DOI: 10.7717/peerj.17194/supp-1

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Mesut Yilmaz conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, contributed reagents, materials, analysis tools, and approved the final draft.

Tulin Arslan conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, contributed reagents, materials, analysis tools, and approved the final draft.

Mükerrem Atalay Oral conceived and designed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Aysegul Kubilay conceived and designed the experiments, performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, contributed reagents, analysis tools, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Akdeniz University Animal Experiments Local Ethics Committee and their ethical compliance was approved with the protocol number 737/2018.03.001.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

The Ministry of Agriculture and Forestry is a government ministry of the Republic of Turkey and approved the study (92190712-605.99-E.5270).

Data Availability

The following information was supplied regarding data availability:

The data is available at NCBI: OK376503.

Funding

The study was financially supported by the Scientific Research Projects Coordination Unit of Akdeniz University under the grant number of FBA-2018-3797. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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