No effects of the antiandrogens cyproterone acetate (CPA), flutamide and p,p’-DDE on early sexual differentiation but CPA-induced retardation of embryonic development in the domestic fowl (Gallus gallus domesticus)

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Dosing

Dissection, tissue preparation and evaluation

Determination of sexual genotype

Determination of embryonic energy metabolism

Measurements and statistics

Results

Embryonic mortality and malformations

Morphological observation of the gonads—gonad surface area

Histological observation of the gonads—left testis and ovary

Discussion

Embryonic mortality and malformations

Morphological observation of the gonads—gonad surface area and left testis and ovary

Overall Conclusions

Supplemental Information

Raw data

All measurements for general or gonad-based endpoints of untreated and substance treated embryos.

DOI: 10.7717/peerj.16249/supp-1

Additional Information and Declarations

Competing Interests

Prof. Dr Jörg Oehlmann is an Academic Editor for PeerJ. Luzie Jessl is an employee of R-Biopharm AG.

Author Contributions

Luzie Jessl conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Jörg Oehlmann conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplementary File.

Funding

This work was carried out in the framework of the project GenOvotox II, funded by the Federal Ministry of Education and Research (BMBF; project no 031A104B). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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