A retrospective study of consistency between immunohistochemistry and polymerase chain reaction of microsatellite instability in endometrial cancer

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Patients and samples

MMR protein IHC staining

DNA extraction

MSI testing using PCR-CE for fragment analysis

Discordant cases evaluated with the Promega panel and NGS test and an additional MLH1 promoter methylation assay

Statistical analysis

Results

Clinicopathological characteristics

MMR/MSI status associations with clinicopathological features.

Substantial agreement between MSI analysis and IHC testing in endometrial cancer

Analysis of discrepant cases in MMR/MSS by alternative methods

Discussion

Conclusions

Supplemental Information

Sequences of primers

MSP, methylation specific PCR; UMSP, unmethylation specific PCR

DOI: 10.7717/peerj.15920/supp-1

Patient demographic information and clinicopathologic features (n = 333)

EC, endometrioid carcinoma; SC, serous carcinoma; CC, clear cell carcinoma; UC, undifferentiated carcinoma of the endometrium; CS, carcinosarcoma; MC, mixed carcinoma; MLA, mesonephric-like adenocarcinomas; FIGI, International Federation of Gynecology and Obstetrics; LVSI, lymphovascular invasion; MMR, mismatch repair; dMMR, mismatch repair deficient; pMMR, mismatch repair proficient; MSI, microsatellite instability; MSS, microsatellite stable; MSI-L, microsatellite instability-low; MSI-H, microsatellite instability-high.

DOI: 10.7717/peerj.15920/supp-2

The results of four MMR protein expressions detected in curettage and hysterectomy specimens

MMR, mismatch repair.

DOI: 10.7717/peerj.15920/supp-3

The MMR or MSI status of curettage and hysterectomy specimens

MMR, mismatch repair; dMMR, mismatch repair deficient; pMMR, mismatch repair proficient; MSI, microsatellite instability; MSI-H, microsatellite instability-high; MSS/MSI-L, microsatellite stable/microsatellite instability-low.

DOI: 10.7717/peerj.15920/supp-4

Raw data of the patients’ essential characteristics and pathological features from medical records

DOI: 10.7717/peerj.15920/supp-5

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Cheng Wang conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Wei Kuang conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Jing Zeng conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Yang Ren analyzed the data, prepared figures and/or tables, and approved the final draft.

Qianqi Liu performed the experiments, prepared figures and/or tables, and approved the final draft.

Huanxin Sun analyzed the data, prepared figures and/or tables, and approved the final draft.

Min Feng analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Dongni Liang analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The study was approved by the Ethics Committee of West China Second University Hospital of Sichuan University Institutional (No.2023037)

Data Availability

The following information was supplied regarding data availability:

The raw data of clinical and pathologic features in 333 endometrial cancers is available in the Supplementary Files.

Funding

The authors received no funding for this work.

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