Rat liver regeneration following ablation with irreversible electroporation

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Introduction

Materials and Methods

Animal subjects

Rat liver irreversible electroporation

Histology

Immunohistochemistry

q-RT-PCR

Results

Pulsed electric fields cause immediate vascular congestion and rapid decellularization in the exposed area

Early events during liver regeneration after IRE ablation

Progenitor cells and hepatocytes contribute to the liver regeneration after PEF ablation

Long-term regeneration of liver lobes following PEF ablation

Discussion

Conclusions

Supplemental Information

Original figures in ppt format.

DOI: 10.7717/peerj.1571/supp-1

Additional Information and Declarations

Competing Interests

B.E.U. has a financial interest in Organ Solutions, LLC, that is reviewed and arranged by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies. The rest of the authors of this manuscript have no conflicts of interest to disclose.

Author Contributions

Alexander Golberg conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Bote G. Bruinsma conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Maria Jaramillo conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Martin L. Yarmush conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Basak E. Uygun conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of the Massachusetts General Hospital and were kept in accordance with the guidelines of the National Research Council: Animal protocol #2013N000127.

Data Deposition

The following information was supplied regarding data availability:

The data generated in this research is supplied as a Supplemental Dataset file.

Funding

This work was supported by the Claflin Distinguished Scholar Award from Massachusetts General Hospital (2013A050726) to Basak E. Uygun. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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