DNAJ heat shock protein family member C1 can regulate proliferation and migration in hepatocellular carcinoma

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Material and Methods

Data collection

DNAJC1 expression analysis

Prognostic role of DNAJC1 in HCC

Gene set enrichment analysis (GSEA)

Immunohistochemistry

Cell culture

RNA extraction and quantitative real-time PCR

Western blotting

Transfection

Cell counting kit-8 (CCK-8) assay

Colony formation assay

Wound healing assay

Transwell migration and invasion assays

Hoechst 33342 staining 

Flow cytometry

Statistical analysis

Results

Expression levels of DNAJC1 in HCC

Prognostic role of DNAJC1 in HCC

Validation of DNAJC1 expression levels

Expression of DNAJC1 mRNA and protein after siRNA transfection

Knockdown of DNAJC1 reduces the proliferation of HCC cells

DNAJC1 knockdown promotes HCC cell apoptosis

DNAJC1 may mediate HCC cell apoptosis via p53 signaling pathway

Knockdown of DNAJC1 reduces the migration and invasion of HCC cells

DNAJC1 may mediate HCC cell migration through the EMT signaling pathway

Discussion

Conclusions

Supplemental Information

Raw data exported from the Western blot applied for data analyses and preparation for Figs. 2A, 3A, 3B and 6B

DOI: 10.7717/peerj.15700/supp-1

Raw data exported from the Western blot applied for data analyses and preparation for Figs. 6C, 9A and 9B

DOI: 10.7717/peerj.15700/supp-2

Numeric data from Western Blots

DOI: 10.7717/peerj.15700/supp-3

Human participant information

DOI: 10.7717/peerj.15700/supp-4

Additional Information and Declarations

Competing Interests

All authors declare that they have no financial, personal interests or beliefs that affect their objectivity, and have no economic or personal relationships with other people or organizations that improperly influence or prejudice their works.

Author Contributions

Yu-Chun Fan conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Zhi-Yong Meng conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Chao-Sheng Zhang performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

De-Wei Wei performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Wan-Shuo Wei performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Xian-Dong Xie performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Ming-Lu Huang performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Li-He Jiang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All procedures were performed according to the Ethical Guidelines for Human Genome/Gene Research and were approved by the Ethics Committee of Affiliated Hospital of Youjiang Medical College for Nationalities (2022090501).

Data Availability

The following information was supplied regarding data availability:

The data is available at GitHub and Zenodo: https://github.com/Yu-ChunFan/Peer-J/tree/V1.1.2.

Yu-ChunFan. (2023). Yu-ChunFan/Peer-J: V1.1.2 (V1.1.2). Zenodo. https://doi.org/10.5281/zenodo.7962889

Funding

This research was supported by the Research project on high-level talents of Youjiang Medical College for Nationalities (Grant No. YY2021SK02), the Foundation of Nanning Qingxiu District Key Research and Development Project (Grant No. 2020023), the Key Laboratory of Minimally Invasive Techniques & Rapid Rehabilitation of Digestive System Tumor of Zhejiang Province (Grant No. 21SZDSYS13) Open Fund from Key Laboratory of Cellular Physiology (Shanxi Medical University), Ministry of Education, China (Grant No. CELLPHYSIOL/SXMU-2021-08), the Grant of National-level project of university students’ innovation and entrepreneurship in 2022 (Grant No. 202210599005; 202210599007) and the Grant of Guangxi provincial-level project of university students’ innovation and entrepreneurship in 2022 (Grant No. S202210599056). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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