New seed coating containing Trichoderma viride with anti-pathogenic properties

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New seed coating containing Trichoderma viride with anti-pathogenic properties @thePeerJ https://t.co/MpQoJ3bJFZ https://t.co/JgVyJ9SU3G
Plant Biology

Main article text

 

Introduction

Materials and Methods

Microorganisms

In vitro antagonistic activity of T. viride strains against plant pathogens

where C is the radial growth of plant pathogen (mm) when grown without T. viride (control), and T is the radial growth of plant pathogen (mm) in the presence of T. viride strains.

Seed coating

Impact of seed coating on B. napus germination and seedling growth

where N is the number of total seeds completed germination at the end of the experiment and S is the number of initial seeds used;

where G is the percentage of seed germination at 1-day intervals, and t is the total germination period;

where N is the germination count on any counting period, and T is the time point in days until the last day (x).

Determination of SOD activity

Expression analysis of BnRSH genes

Statistical analyses

Results

The effect of T. viride on the growth of pathogenic fungi

The effect of seed coating on seed germination and seedling growth

The effect of seed coating on BnRSH gene expression

The effect of seed coating on SOD activity

Discussion

The new seed coating has the potential to improve plant immunity to fungal pathogens

The new materials for seed coating promote plant growth

The developed seed coating does not induce stress in plants

Conclusions

Supplemental Information

Photographs of fungal inhibition by Trichoderma.

Images depict comparative growth of multiple fungal variants (labelled), thereby indicating the inhibitory potential of Trichoderma.

DOI: 10.7717/peerj.15392/supp-1

Six-day-old seedlings grown from uncoated and coated seeds.

1 - uncoated seeds, 2 - methylcellulose-chitin-Trichoderma viride I, 3 - methylcellulose-chitin-T. viride II, 4 - methylcellulose, 5 - chitin, 6 - Methylcellulose–chitin, 7 - T. viride I, and 8 - T. viride II.

DOI: 10.7717/peerj.15392/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Sena Turkan performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Agnieszka Mierek-Adamska analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Milena Kulasek performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Wiktoria B Konieczna performed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Grażyna B Dąbrowska conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The data is available at Zenodo: Sena Turkan, Agnieszka Mierek-Adamska, Milena Kulasek, Wiktoria Beata Konieczna, & Grażyna Barbara Dąbrowska. (2022). Seed coating for Brassica napus L. [Data set]. Zenodo. https://doi.org/10.5281/zenodo.7628268.

Funding

This work was supported by Nicolaus Copernicus University under the Excellence Initiative-Research University programme (IDUB), competition “Grants4NCUStudents”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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