Liver proteomic analysis reveals the key proteins involved in host immune response to sepsis

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Ethics statement

Sepsis mouse models

Sample and protein preparation

TMT labeling

HPLC fractionation

LC-MS/MS analysis

Proteomics data analysis

Parallel reaction monitoring (PRM) for verification

Statistical analysis

Results

Proteomic data statistics

Analysis of differentially abundant proteins between sepsis model and control

Functional characterization and enrichment of DEPs

Protein interaction network for DEPs

Discussion

Conclusions

Supplemental Information

The identified information of differentially expressed significant proteins by TMT.

DOI: 10.7717/peerj.15294/supp-1

GO enrichment analysis of upregulated DEPs.

DOI: 10.7717/peerj.15294/supp-2

GO enrichment analysis of downregulated DEPs.

DOI: 10.7717/peerj.15294/supp-3

Comparision of targeted protein expression according to TMT and PRM approaches.

DOI: 10.7717/peerj.15294/supp-4

Author_Checklist_-_Full.

DOI: 10.7717/peerj.15294/supp-5

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Yingying Chen conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Hui Gong performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Donge Tang performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Lan Yu analyzed the data, prepared figures and/or tables, and approved the final draft.

Shoubin Long performed the experiments, prepared figures and/or tables, and approved the final draft.

Bao Zheng performed the experiments, prepared figures and/or tables, and approved the final draft.

Dixian Luo conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Anji Cai conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All experiments and procedures in this study were performed in strict accordance with protocols approved by the Institutional Animal Care and Use Committee of Shenzhen People’s Hospital Laboratory Animal Center (approval number: AUP-220516-TDE-0341-01).

Data Availability

The following information was supplied regarding data availability:

The mass spectrometry proteomics data are available at the ProteomeXchange Consortium via the iProX partner repository: PXD037868.

The raw measurements are available in the Supplemental Files.

Funding

This work was supported by the Shenzhen Nanshan District Scientific Research Program of the People’s Republic of China (No. 2020114) and the Natural Science Foundation of Guangdong Province (No. 2020A1515011303). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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