Effects of leptin on the viability of human ovarian cancer cells and changes in cytokine expression levels

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Reagents

Cells and culture

XTT cell viability assay

Cytokine array

Statistical analysis

Results

Effects of Leptin on cell viability of OVCAR-3 and MDAH-2774 cells

Changes in the cytokine levels after leptin exposure in ovarian cancer cells

Discussion

Supplemental Information

Cytokin array layout.

DOI: 10.7717/peerj.15246/supp-1

Cytokin array datasheet.

DOI: 10.7717/peerj.15246/supp-2

MDAH and leptin treatment.

DOI: 10.7717/peerj.15246/supp-3

MDAH cell control group.

DOI: 10.7717/peerj.15246/supp-4

Leptin treatment in ovcar cell line; cytokin array results.

DOI: 10.7717/peerj.15246/supp-5

Leptin treatment in mdah cell line; cytokin array results.

DOI: 10.7717/peerj.15246/supp-6

Ovcar 3 and leptin treatment.

DOI: 10.7717/peerj.15246/supp-7

OVCAR 3 cell control group.

DOI: 10.7717/peerj.15246/supp-8

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Fatih Dincer conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Harika Atmaca conceived and designed the experiments, performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Levent Akman conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Latife Merve Oktay performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Burcak Karaca performed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Mustafa Cosan Terek analyzed the data, prepared figures and/or tables, and approved the final draft.

Microarray Data Deposition

The following information was supplied regarding the deposition of microarray data:

Raybiotech, GA, USA.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplemental Files.

Funding

This work was supported by Ege University Scientific Research Project No: 10340. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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