Identification and biodiversity patterns of Aspergillus species isolated from some soil invertebrates at high altitude using morphological characteristics and phylogenetic analyses

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Identification and biodiversity patterns of Aspergillus species isolated from some soil invertebrates at high altitude using morphological characteristics and phylogenetic analyses Read the full article https://t.co/RUPqVgXC6X #AgriculturalScience #Microbiology #Mycology
Identification and biodiversity patterns of Aspergillus species isolated from some soil invertebrates at high altitude using morphological characteristics and phylogenetic analyses Read the full article https://t.co/WQNyya3o3H #AgriculturalScience #Microbiology #Mycology
Microbiology

Main article text

 

Introduction

Materials and Methods

Fungal isolation

Morphological identification of fungal isolates

DNA extraction

PCR amplification of ITS region and TEF gene

Sequencing analysis of ITS region and TEF gene

Molecular detection of aflatoxin and ochratoxin-producing genes

Inter simple sequence repeats (ISSR)-PCR analysis

Data analysis

Results

Isolation of Aspergillus species

Morphological identification

Molecular identification of Aspergillus species using 5.8S-ITS region sequence

Molecular identification of Aspergillus species based on TEF-1 α DNA gene

ISSR-PCR analysis

Genetic distances and the cluster dendrogram

Detection of aflatoxin and ochratoxin-producing genes

Discussion

Conclusion

Supplemental Information

Phylogenetic tree (with bootstrap) and diversity of the 5.8S-ITS region with different Aspergillus species compared with reference Aspergillus strains

DOI: 10.7717/peerj.15035/supp-1

Phylogenetic tree (with bootstrap) and diversity of the TEF-1 α gene with different Aspergillus species compared with reference Aspergillus strains

DOI: 10.7717/peerj.15035/supp-2

PCR amplification of some mycotoxin genes in studied Aspergillus species, where A is Gdh gene, B is Aopks, C is Omt-A and D is Afl-A gene

DOI: 10.7717/peerj.15035/supp-3

Sequence of ITS of Aspergillus isolates

DOI: 10.7717/peerj.15035/supp-4

Sequence of Tef gene of Aspergillus isolates

DOI: 10.7717/peerj.15035/supp-5

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Mohamed Fadl Awad conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Bander Albogami conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Tarombera Mwabvu conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Montaser M. Hassan conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Alaa Baazeem conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Mohamed M. Hassan conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Mohsen Mohamed Elsharkawy conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The sequences are available at GenBank: ON160831ON160840.

Data Availability

The following information was supplied regarding data availability:

The raw data is available in the Supplementary Files.

Funding

This research was funded by the Dean of Scientific Research through the High-Altitude Research Centre at Taif University, Taif, Saudi Arabia, under project number 1-442-43. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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